MABT217

Anti-Calreticulin Antibody, clone 16B11.1

clone 16B11.1, from mouse

• Synonyme(s) : Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 16B11.1, monoclonal
• Espèces réactives: human
• Technique(s): flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_004334
• Numéro d'accès UniProt: P27797
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CALR(811)

Description

Description générale

Calreticulin is a molecular Ca+ binding chaperone promoting folding, oligomeric assembly, and quality control in ER. Calreticulin is a highly versatile lectin-like chaperone, and it participates during the synthesis of a variety of molecules, including ion channels, surface receptors, integrins and transporters. Calreticulin indeed provides up to 45% of the Ca2+-buffering capacity for a pool of the IP3-sensitive Ca2+ inside the ER. Calreticulin also functions in cell adhesion, integrin-dependent Ca2+ signaling and steroid-sensitive gene expression.

Immunogène

GST-tagged recombinant protein corresponding to human Calreticulin.

Application

Immunohistochemistry Analysis: A 1:300-1,000 dilution from a representative lot detected Calreticulin in human colorectal cancer and human tonsil tissues.

Flow Cyometry Analysis: A 1:500 dilution from a representative lot detected Calreticulin in non serum starved HeLa cells.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-Calreticulin Antibody, clone 16B11.1 is validated for use in Western Blotting, IHC, Flow Cytometry for the detection of Calreticulin.

Qualité

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calreticulin on 10 µg of HeLa cell lysate.

Description de la cible

~48 kDa observed

Liaison

Replaces: MABT145

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable