MABN2406

Anti-Tau Antibody, clone TNT-2

clone TNT-2, from mouse

• Synonyme(s) : EC:4.6.1.2, Guanylate cyclase 2D, retinal, Guanylate cyclase E (GC-E), Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, RETGC-1, ROS-GC, Retinal guanylyl cyclase 1, Rod outer segment membrane guanylate cyclase
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: TNT-2, monoclonal
• Poids mol.: calculated mol wt 120.37 kDa; observed mol wt ~N/A kDa
• Produit purifié par: using protein G
• Espèces réactives: rat, human, mouse
• Conditionnement: antibody small pack of 100
• Technique(s): ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Séquence de l'épitope: C-terminal
• Numéro d'accès NCBI: NP_058519
• Numéro d'accès UniProt: P10636
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MAPT(4137) ; mouse ... Mapt(17762) ; rat ... Mapt(29477)

Description

Description générale

Microtubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Tau proteins are mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. Normally, Tau promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The localization of Tau protein in the Alzheimer s disease (AD) brain is markedly abnormal and may contribute to neuronal dysfunction. In AD, normal soluble cytoskeletal elements, such as Tau and neurofilaments, are transformed into insoluble paired helical filaments (PHFs). This is linked to the post-translational change in Tau, primarily its hyperphosphorylation by a number of protein kinases. In its non-phosphorylated state, Tau promotes rapid and extensive microtubule (MT) polymerization. A significant consequence of Tau hyperphosphorylation in AD is a reduction in its ability to bind microtubules and promote microtubule assembly. Hyperphosphorylated Tau may contribute to a destabilized microtubule network, impaired axonal transport, and ultimately result in NFT formation and neuronal death.

Spécificité

Clone TNT-2 specirfically detects tau in human, mouse, and rat brain. It targets a sequence of 17 amino acids from the N-terminal region that is common to all isoforms (1-6) of tau.

Immunogène

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of human Tau.

Application

Quality Control Testing

Evaluated by Immunohistochemistry in mouse retina tissue section

Immunohistochemistry (Paraffin) Analysis (IHC): A 1:50 dilution from a representative lot detected Guanylate cyclase 1 in mouse retina tissue sections.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Guanylate cyclase 1 in Immunofluorescence application (Ying, G., et al. (2018). J Biol Chem. 293(45):17546-17558).

Immunohistochemistry Analysis: A representative lot detected Guanylate cyclase 1 in Immunohistochemistry application (Haire, S.E., et al. (2006). Invest Ophthalmol Vis Sci. 47(9):3745-53).

Western Blotting Analysis: A representative lot detected Guanylate cyclase 1 in Western Blotting application (Haire, S.E., et al. (2006). Invest Ophthalmol Vis Sci. 47(9):3745-53).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Qualité

Evaluated by Western Blotting in rat entorhinal cortex.

Western Blotting Analysis: 0.02 µg/mL of this antibody detected Tau in 10 µg of rat entorhinal cortex lysate.

Description de la cible

~55 kda Observed. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1