MABE281
Anti-MDM2 Antibody, clone 2A10
clone 2A10, from mouse
Synonyme(s) :
E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
culture supernatant
Type de produit anticorps
primary antibodies
Clone
2A10, monoclonal
Espèces réactives
human
Technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... MDM2(4193)
Description générale
MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes the transcriptional activity of p53 in two main ways. The N-terminal of MDM2 interacts and disables the transactivation domain of p53. In addition, the C-terminal E3 ligase region of MDM2 can attach monoubiquitin to p53 resulting in the transport of p53 from the nucleus to the cytoplasm, thereby preventing the interaction of p53 with target DNA sequences. In addition, MDM2 may also attach polyubiquitin chains to p53, resulting in the degradation of p53 by the proteosome. MDM2 may produce these effects in cooperation with the Mdmx protein. Overall, MDM2 inhibits the ability of p53 to induce cell cycle arrest or apoptosis, and may contribute to oncogenesis.
Application
Anti-MDM2 Antibody, clone 2A10 is a Mouse Monoclonal Antibody for detection of MDM2 also known as E3 ubiquitin-protein ligase Mdm2 or Hdm2 & has been validated in WB & IP.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in human lymphoblastoid cell line (NL553) treated with NCS. (Khosravi, R., et al. (1999). PNAS. 96(26):14973–14977.)
Qualité
Evaluated by Western Blot in A-549 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected MDM2 on 10 µg of A-549 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected MDM2 on 10 µg of A-549 cell lysate.
Description de la cible
~90 kDa observed. The calculated molecular weight is 55 kDa, however MDM2 has been shown as a ~90 kDa band in western blots (Erhardt, P., et al. (1997). The Journal of Biological Chemistry. 272:15049-15052.). Uniprot describes many isoforms between 12-56 kDa, which can be highly phosphorylated and ubiquitinated.
Forme physique
Format: Purified
Purified mouse monoclonal IgG2aκ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Remarque sur l'analyse
Control
A-549 cell lysate
A-549 cell lysate
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Susie Lee et al.
PloS one, 5(6), e10995-e10995 (2010-06-12)
Cell proliferation in all rapidly renewing mammalian tissues follows a circadian rhythm that is often disrupted in advanced-stage tumors. Epidemiologic studies have revealed a clear link between disruption of circadian rhythms and cancer development in humans. Mice lacking the circadian
J-L Roh et al.
Cell death & disease, 4, e956-e956 (2013-12-18)
The tumor suppressor p53 is often inactivated in head and neck cancer (HNC) through TP53 mutations or overexpression of mouse double minute 2 or mouse double minute X. Restoration of p53 function by counteracting these p53 repressors is a promising
Chui Chui Ho et al.
Cancer research, 66(4), 2233-2241 (2006-02-21)
Stalled replication forks induce p53, which is required to maintain the replication checkpoint. In contrast to the well-established mechanisms of DNA damage-activated p53, the downstream effectors and upstream regulators of p53 during replication blockade remain to be deciphered. Hydroxyurea triggered
Saeed Arefanian et al.
Oncoimmunology, 5(12), e1238543-e1238543 (2017-01-27)
Individuals with robust natural killer (NK) cell function incur lower rates of malignancies. To expand our understanding of genetic factors contributing to this phenomenon, we analyzed NK cells from cancer resistant and susceptible strains of mice. We identified a correlation
Muktar A Mahajan et al.
Molecular endocrinology (Baltimore, Md.), 21(8), 1822-1834 (2007-05-31)
Nuclear receptor coregulator (NRC) is a 250-kDa nuclear protein involved in transcriptional activation of nuclear hormone receptors, nuclear factor-kappaB, c-Jun, c-Fos, and cAMP response element-binding protein. NRC is organized into a modular structure consisting of two activation domains (AD1 and
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