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Key Documents

MAB8052

Sigma-Aldrich

Anti-Adenovirus Antibody, clone 20/11

clone 20/11, Chemicon®, from mouse

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Clone

20/11, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

Isotype

IgG1

Conditions d'expédition

wet ice

Description générale

Adenoviruses are 65-80 nm, non-enveloped, regular icosahedron pathogens often associated with respiratory and gastrointestinal illness as well as conjunctivitis. Well over 40 types of adenoviruses have currently been recognized. In addition to their deleterious effects, adenoviruses have been used in vaccine production and in gene therapy. They also have the ability to trigger cell proliferation by interfering with host cellular anti-oncogenes. Since they are easy to prepare, multiple quickly and efficiently, and have the ability to infect and deliver their genome (including researcher-created constructs) to the nucleus of a variety of post-mitotic cells, adenoviruses are extremely valuable in research and medical applications ranging from virus/host cell interaction to the insertion of novel genes or regulatory mechanisms into eukaryotic cellular systems, to delivery of therapeutic anti-cancer genes.

Spécificité

Recognizes All 41 serotypes of Adenovirus.

Immunogène

Adeno 3.

Application

Anti-Adenovirus Antibody, clone 20/11 detects level of Adenovirus & has been published & validated for use in ELISA, FC, IF, IH, IH(P).
EIA, IFA

Immunohistochemistry: 1:200. In trypsinized (with 0.1% trypsin in Tris buffer pH 7.6 containing 0.1% Calcium Cloride; for 5-10 minutes at room temp.) paraffin embedded tissue. Do not use antigen retrieval.

Flow cytometry.

Final working dilutions and protocols must be determined by end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Forme physique

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Stockage et stabilité

Maintain at +2–8°C in convenient aliquots for up to 12 months. Do not store in a diluted format.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Remarque sur l'analyse

Control
Adenovirus Control Slides, Catalogue Number 5009-5

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Efficient internalization into low-passage glioma cell lines using adenoviruses other than type 5: an approach for improvement of gene delivery to brain tumours.
Skog, J; Edlund, K; Widegren, B; Salford, LG; Wadell, G; Mei, YF
The Journal of General Virology null
Adenovirus types 11p and 35 attach to and infect primary lymphocytes and monocytes, but hexon expression in T-cells requires prior activation.
Anna Segerman, Kristina Lindman, Ya-Fang Mei, Annika Allard, Goran Wadell
Virology null
Simultaneous occurrence of lymphoepithelial cysts, cytomegalovirus and mycobacterial infections in the intraparotid lymph nodes of a patient with AIDS.
P A Vargas, H Villalba, A P Passos, P H Saldiva, T Mauad, H H Caiaffa Filho, A Lucena, O P Almeida
Journal of Oral Pathology & Medicine null
Adenovirus type 7 induces interleukin-8 in a lung slice model and requires activation of Erk.
Booth, JL; Coggeshall, KM; Gordon, BE; Metcalf, JP
Journal of virology null
David Marchant et al.
Journal of virology, 84(21), 11359-11373 (2010-08-13)
Respiratory viruses exert a heavy toll of morbidity and mortality worldwide. Despite this burden there are few specific treatments available for respiratory virus infections. Since many viruses utilize host cell enzymatic machinery such as protein kinases for replication, we determined

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