MAB5406B
Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate
clone 1G10.2, from mouse, biotin conjugate
Synonyme(s) :
Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
biotin conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
1G10.2, monoclonal
Espèces réactives
rat
Réactivité de l'espèce (prédite par homologie)
human (based on 100% sequence homology), mouse (based on 100% sequence homology)
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
Isotype
IgG2a
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... GAD1(2571)
Description générale
Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, 1999). GAD65 is an amphiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk, 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
Spécificité
Reacts with GAD67. No detectable cross reactivity with GAD65 by Western blot on rat brain lysate.
Immunogène
Recombinant GAD67 protein
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAD67 in rat E18 primary cortex cells.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
This Anti-GAD Antibody, clone 1G10.2, Biotin Conjugate is validated for use in IHC for the detection of GAD67. See below for more information about this anti GAD antibody.
Qualité
Evaluated by Immunohistochemistry in rat cortex tissue.
Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected GAD67 in rat cortex tissue.
Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected GAD67 in rat cortex tissue.
Description de la cible
~67 kDa
Forme physique
Protein A purified
Purified mouse monoclonal IgG2a conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Stockage et stabilité
Maintain refrigerated at 2-8 °C in undiluted aliquots for up to 6 months from date of receipt.
Remarque sur l'analyse
Control
Rat cortex tissue
Rat cortex tissue
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Jose A Fernandez-Leon et al.
eLife, 10 (2021-12-17)
The recollection of environmental cues associated with threat or reward allows animals to select the most appropriate behavioral responses. Neurons in the prelimbic (PL) cortex respond to both threat- and reward-associated cues. However, it remains unknown whether PL regulates threat-avoidance
Jean-Christophe Delpech et al.
Science translational medicine, 13(611), eabe8455-eabe8455 (2021-09-16)
Abnormally phosphorylated tau, an early neuropathologic marker of Alzheimer’s disease (AD), first occurs in the brain’s entorhinal cortex layer II (ECII) and then spreads to the CA1 field of the hippocampus. Animal models of tau propagation aiming to recapitulate this
V Korzhova et al.
Communications biology, 4(1), 1368-1368 (2021-12-09)
Alzheimer's disease (AD) is associated with aberrant neuronal activity, which is believed to critically determine disease symptoms. How these activity alterations emerge, how stable they are over time, and whether cellular activity dynamics are affected by the amyloid plaque pathology
Zhi Ruan et al.
Brain : a journal of neurology, 144(1), 288-309 (2020-11-28)
Extracellular vesicles are highly transmissible and play critical roles in the propagation of tau pathology, although the underlying mechanism remains elusive. Here, for the first time, we comprehensively characterized the physicochemical structure and pathogenic function of human brain-derived extracellular vesicles
Hao-Yu Zou et al.
The Journal of clinical investigation, 132(8) (2022-04-16)
Mushroom spine loss and calcium dyshomeostasis are early hallmark events of age-related neurodegeneration, such as Alzheimer's disease (AD), that are connected with neuronal hyperactivity in early pathology of cognitive brain areas. However, it remains elusive how these key events are
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