MAB382
Anti-Myelin Basic Protein Antibody
CHEMICON®, mouse monoclonal, 1
Synonyme(s) :
Myelin A1 protein, Myelin membrane encephalitogenic protein, myelin basic protein
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
product name
Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1, culture supernatant, clone 1, Chemicon®
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
culture supernatant
Type de produit anticorps
primary antibodies
Clone
1, monoclonal
Espèces réactives
bovine, rat, rabbit (weakly), human
Ne doit pas réagir avec
guinea pig
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
western blot: suitable
Isotype
IgG2a
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... MBP(4155)
rat ... Mbp(24547)
Description générale
The classic group of MBP isoforms (isoforms 4-14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Isoform 1 Golli-MBP1, HOG7, 33 kDa
Isoform 2 Golli-MBP2, HOG5, 21.5 kDa
Isoform 3 MBP1, 21.5 kDa
Isoform 4 MBP2, 20.2 kDa
Isoform 5 MBP3, 18.5 kDa
Isoform 6 MBP4, 17.2 kDa
(SP_P02686)
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Isoform 1 Golli-MBP1, HOG7, 33 kDa
Isoform 2 Golli-MBP2, HOG5, 21.5 kDa
Isoform 3 MBP1, 21.5 kDa
Isoform 4 MBP2, 20.2 kDa
Isoform 5 MBP3, 18.5 kDa
Isoform 6 MBP4, 17.2 kDa
(SP_P02686)
Spécificité
Reacts with MBP from human, bovine and rat, epitope 129-138
Immunogène
Bovine myelin basic protein
Epitope: a.a. 129-138
Application
Immunohistochemistry(paraffin) Analysis:
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistology on frozen sections at 1:10
Western Blot Analysis:
A previous lot of this antibody was used in Western Blot.
ELISA:
A 1:200-1:1,000 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in Radioimmunoassay.
Optimal working dilutions must be determined by end user.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistology on frozen sections at 1:10
Western Blot Analysis:
A previous lot of this antibody was used in Western Blot.
ELISA:
A 1:200-1:1,000 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in Radioimmunoassay.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
This Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1 is validated for use in ELISA, IH, IH(P), RIA, WB for the detection of Myelin Basic Protein.
Qualité
Routinely evaluated by immunohistochemistry on brain tissue.
Immunohistochemistry(paraffin) Analysis:
MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistochemistry(paraffin) Analysis:
MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Description de la cible
19 kDa
Forme physique
Culture supernatant containing 0.2 M Tris/HCl, pH 7.4 with 5-10% fetal calf serum and 0.1% sodium azide
Unpurified
Stockage et stabilité
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Remarque sur l'analyse
Control
Brain tissue
Brain tissue
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Autoantibodies to myelin basic protein catalyze site-specific degradation of their antigen.
Ponomarenko, NA; Durova, OM; Vorobiev, II; Belogurov, AA; Kurkova, IN; Petrenko et al.
Proceedings of the National Academy of Sciences of the USA null
Nucleus-localized 21.5-kDa myelin basic protein promotes oligodendrocyte proliferation and enhances neurite outgrowth in coculture, unlike the plasma membrane-associated 18.5-kDa isoform.
Smith, GS; Samborska, B; Hawley, SP; Klaiman, JM; Gillis, TE; Jones, N; Boggs, JM; Harauz, G
Journal of Neuroscience Research null
Michel Guipponi et al.
The American journal of pathology, 171(2), 608-616 (2007-07-11)
Defective proteolysis has been implicated in hearing loss through the discovery of mutations causing autosomal recessive nonsyndromic deafness in a type II transmembrane serine protease gene, TMPRSS3. To investigate their physiological function and the contribution of this family of proteases
Vibol Chhor et al.
Brain, behavior, and immunity, 63, 197-209 (2016-11-08)
The cognitive and behavioural deficits caused by traumatic brain injury (TBI) to the immature brain are more severe and persistent than TBI in the mature brain. Understanding this developmental sensitivity is critical as children under four years of age sustain
Michael J Whitehead et al.
Scientific reports, 8(1), 5219-5219 (2018-03-28)
Axon degeneration underlies many nervous system diseases; therefore understanding the regulatory signalling pathways is fundamental to identifying potential therapeutics. Previously, we demonstrated heparan sulphates (HS) as a potentially new target for promoting CNS repair. HS modulate cell signalling by both
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