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HGAMMAG-301K

Millipore

MILLIPLEX® Human Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples.

Synonyme(s) :

Human Ig isotype multiplex panel, Human immune globulin multiplex panel, Human immunoglobulin multiplex kit, Luminex® human immunoglobulin immunoassay, Millipore Human immunoglobulin panel

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.47

Niveau de qualité

200

Espèces réactives

human

Fabricant/nom de marque

Milliplex®

assay range

accuracy: 88-99%
sensitivity: 0.08 ng/mL
(IgG4)
sensitivity: 0.09 ng/mL
(IgG3)
sensitivity: 0.47 ng/mL
(IgM)
sensitivity: 1.5 ng/mL
(IgA)
sensitivity: 10 ng/mL
(IgG1)
sensitivity: 85 ng/mL
(IgG2)
standard curve range: 0.2-150 ng/mL
(IgG3)
standard curve range: 0.4-300 ng/mL
(IgG4)
standard curve range: 14-10,000 ng/mL
(IgG1)
standard curve range: 2.1-1,500 ng/mL
(IgA)
standard curve range: 3.4-2,500 ng/mL
(IgM)
standard curve range: 41-30,000 ng/mL
(IgG2)

Technique(s)

multiplexing: suitable

Méthode de détection

fluorometric (Luminex xMAP)

Conditions d'expédition

wet ice

Catégories apparentées

Description générale

Produced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens, and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Five major isotypes have been identified in placental mammals: IgM, IgG, IgA, IgE and IgD (B-cell receptor) – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions, and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes. In addition, isotyping applications include analyzing hybridomas during antibody generation and vaccine development.

The MILLIPLEX® Human Isotyping Bead Panel, measuring human IgG subclasses (1, 2, 3, 4), IgM and IgA is designed to enable you to measure both classes and subclasses accurately – all in one well. In addition, this panel allows you to choose only those immunoglobulins you need for your experiments.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Application

  • Analytes: IgG1, IgG2, IgG3, IgG4, IgA, IgM
  • Recommended Sample type: Serum, plasma, or tissue/cell lysate and culture supernatant samples
  • Recommended Sample dilution: 1:16,000 diluted plasma or serum, or cell culture supernatant diluted to approximately 1μg/mL Ig per well
  • Assay Run Time: One day
  • Research Category: Inflammation & Immunology

Caractéristiques et avantages

Design your multiplex kit by choosing available analytes within this panel.

Composants

MILLIPLEX MAP Anti-Human κ and λ Light Chain, PE

MILLIPLEX MAP Human Multi-Immunoglobulin Standard

MILLIPLEX MAP Human Immunoglobulin Positive Control

MILLIPLEX MAP Assay Buffer

MILLIPLEX MAP Wash Buffer, 10X

Informations légales

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Organes cibles

Respiratory Tract

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Jiong Wang et al.
Frontiers in immunology, 12, 696370-696370 (2021-08-14)
The COVID-19 pandemic is caused by SARS-CoV-2, a novel zoonotic coronavirus. Emerging evidence indicates that preexisting humoral immunity against other seasonal human coronaviruses (HCoVs) plays a critical role in the specific antibody response to SARS-CoV-2. However, current work to assess
Patricia S Grace et al.
Frontiers in immunology, 12, 679973-679973 (2021-07-23)
With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases. Humoral immunity following TB treatment is largely uncharacterized, and antibody profiling could provide insights into disease
Brian K McFarlin et al.
Journal of dietary supplements, 10(3), 171-183 (2013-08-10)
Strenuous exercise, such as running a marathon, is known to suppress mucosal immunity for up to 24 hr, which can increase the risk of developing an upper respiratory tract infection (URTI) and reduced performance capacity (Allgrove JE, Geneen L, Latif
Sarah H Pedersen et al.
Clinical nutrition (Edinburgh, Scotland), 35(4), 871-879 (2015-06-18)
Understanding mature breastmilk immunology may benefit infants chronically exposed to infectious pathogens in resource-limited regions. This prospective rural/semi-rural Tanzanian cohort of women (n = 102 at delivery; 38% HIV-positive) and their infants (n = 102) investigated breastmilk, maternal and infant serum immunoglobulins (IgA/IgG1-4/IgM) and
Kristin L Boswell et al.
PLoS pathogens, 10(1), e1003853-e1003853 (2014-02-06)
The interaction between follicular T helper cells (TFH) and B cells in the lymph nodes and spleen has a major impact on the development of antigen-specific B cell responses during infection or vaccination. Recent studies described a functional equivalent of
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