ES-006
EmbryoMax® Gelatin Solution
0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.
Synonyme(s) :
Porcine Gelatin Coating Solution, porcine gelatin
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Nom du produit
EmbryoMax® 0.1% Gelatin Solution, The EmbryoMax 0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.
form
liquid
manufacturer/tradename
Specialty Media, EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
Application
1. Add the appropriate amounts of 0.1% Gelatin in Sterile Water to each well of the plate as indicated in the table below:
| Wells | _ | Amount/Well |
|---|---|---|
| 96 | 100 μL | |
| 48 | 300 μL | |
| 24 | 0.5 mL | |
| 12 | 1.0 mL | |
| 6 | 1.5-2.0 mL |
2. Leave the gelatin solution on the wells for at least 30 minutes at room temperature, with dish lids on.
3. Remove the gelatin solution and discard.
4. Immediately add cells to the dish.
Note: Do not allow the dishes to dry before adding cells.
Physical form
Preparation Note
Legal Information
Disclaimer
Classe de stockage
12 - Non Combustible Liquids
wgk
nwg
flash_point_c
Not applicable
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Contenu apparenté
The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.
"As transgenic mouse core laboratories know well, mouse models are a powerful means of determining the function of mammalian genes in the context of whole organisms—especially since over 95% of the mouse genome is similar to the human genome. However, you may not know that: 1. Laboratory mouse strains are descended from ancestors in modern-day Pakistan. 2. The first inbred strain of lab mouse was created in 1909. It was named DBA for its coat color: dilute brown non-agouti. 3. There is a mutant mouse actually named“Bad Hair Day” (Bhrd). Someone get this little guy a stylist! 4. Male mice, when treated with anticancer drugs, have been shown to pass on DNA instability to their offspring. 5. The founder of Jackson Laboratories, C. C. Little, once had lunch with Walt Disney to request that an animated film be created linking Mickey Mouse and the laboratory mouse. The request was denied."
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| ES-006-B | 08436037123900 |