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Merck
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Principaux documents

DC03L

Sigma-Aldrich

Goat Anti-Rabbit IgG Peroxidase Conjugate

lyophilized, Calbiochem®

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

lyophilized

Ne contient pas

preservative

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Immunoaffinity purified goat polyclonal antibody adsorbed against human serum proteins and conjugated to horseradish peroxidase. Recognizes rabbit IgG.
This Goat Anti-Rabbit IgG Peroxidase Conjugate is validated for use in ELISA, Immunohistochemistry for the detection of Rabbit IgG.

Application

ELISA (1:5000-1:100,000)

Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)

Immunohistochemistry (1:500-1:5000)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 250 mM NaCl, 10 mM sodium phosphate, 15 mg/ml BSA, pH 7.6.

Reconstitution

Reconstitute the antibody with 1.5 ml H₂O. Dissolve by gently mixing at 4°C, since extensive mixing will denature antibodies. Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Alternatively, glycerol (to a final concentration of 50%) may be added and the reconstituted antibody then stored at -20°C. Please note, under these conditions the concentration of the antibody and the buffer salts will be one-half of the original concentration.

Autres remarques

To ensure the quality of these reagents each lot is tested with several of our primary antibodies. Antibody specificity is tested by immunoelectrophoresis and shown to be specific for rabbit IgG and light chains common to most rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins has been detected. The antibody has also been tested by ELISA and/or adsorbed to ensure minimal cross-reactivity with human serum proteins, but the antibody may exhibit cross-reactivity with immunoglobulins from other species. Antibody should be titrated for optimal results in individual systems.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3


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Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Alejandro Vicente-Carrillo et al.
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Mayara Caldas Ramos Cunha et al.
PloS one, 8(3), e58872-e58872 (2013-03-22)
Protein malnutrition (PM) results in pathological changes that are associated with peripheral leukopenia, bone marrow (BM) hypoplasia and alterations in the BM microenvironment leading to hematopoietic failure; however, the mechanisms involved are poorly understood. In this context, the BM mesenchymal
Karl A Nath et al.
The American journal of pathology, 166(4), 963-972 (2005-03-29)
Ischemic injury is invoked as a mechanism contributing to end-organ damage and other complications of sickle cell disease (SCD). However, the intrinsic sensitivity of tissues in SCD to ischemic insults has never been addressed. We examined the effect of renal
Bettina P Mihalas et al.
Aging, 11(10), 2968-2997 (2019-05-28)
Oocytes are reliant on messenger RNA (mRNA) stores to support their survival and integrity during a protracted period of transcriptional dormancy as they await ovulation. Oocytes are, however, known to experience an age-associated alteration in mRNA transcript abundance, a phenomenon
Luba Sominsky et al.
Frontiers in neuroscience, 7, 100-100 (2013-06-20)
Neonatal immune challenge by administration of lipopolysaccharide (LPS) produces enduring alterations in the development and activity of neuroendocrine, immune and other physiological systems. We have recently reported that neonatal exposure to an immune challenge by administration of LPS results in

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