APT800

Apoptosis Inducer Set

Apoptosis Inducer Set contains Actinomycin D, Camptothecin, Cycloheximide, Dexamethasone & Etoposide. These compounds can be used for inducing apoptotic activity in many cell culture systems.

• Synonyme(s) : 5 Apotosis Inducers
• UNSPSC Code: 12161503
• NACRES: NA.32
• eCl@ss: 32161000

Propriétés

• Quality Level: 100
• form: liquid
• species reactivity (predicted by homology): mammals
• manufacturer/tradename: Chemicon^®
• technique(s): activity assay: suitable
• shipped in: dry ice

Description

General description

Actinomycin D: Classical anti-neoplastic drug. Cytotoxic inducer of apoptosis against tumor cells. A DNA dependent inhibitor of RNA synthesis, actinomycin promotes induction of apoptosis by some specific stimuli, for example, TRAIL and Fas (CD95). Actinomycin D can also alleviate or block the apoptotic process and decrease the cytotoxicity induced by several stimuli such as the dihydrofolate reductase inhibitor aminopterin and the prostaglandin derivative 15-deoxy-D12,14-prostaglandin J2, thus it can have both pro and ant-apoptotic activities in some systems.



Camptothecin: Powerful anti-tumor agent. Camptoethecin is an inhibitor of DNA-topoisomerase I. The drug activates the p53 resulting in upregulated expression of TRAIL-R2 (DR5) and Bak to overcome TRAIL resistance in Bax-deficient human colon carcinoma cancer cells. In addition, Camptoethecin induced apoptosis can be increased by the presence of cytokines such as TNF via its subsequent inhibition of NFkappa B {Valente P et al Eur J Cancer (2003) 39:1468-77}.



Cycloheximide: A potent protein translation inhibitor which results in the inhibition of protein synthesis and subsequent arrest of cell growth and induction of cell death. Cycloheximide also facilitates apoptosis induction via FasL, however, cycloheximide can also prevent apoptosis in some systems, such as thalassemic serum induction of HUVEC cells {Banjerdpong, R. (1998) Ph.D Thesis Mahidol Univ. Bangkok, Thailand}.



Dexaméthasone : Classic inducer of apoptosis in thymocytes; a glucocorticoid anti-inflammatory agent. Regulates T cell survival, growth and differientiation; modulator of nitric oxide synthase. Induces apoptosis in thymocytes as well as other immune cells like microglia. However, like other apoptotic inducers, dexamethasone can suppress apoptosis in certain systems through the activation of anti-apoptotic proteins such as bcl-X {Chang, et al (1997) FEBS Letter 415:11-15}.



Etoposide: Potent topoisomerase II inhibitor. & Inhibits the cell cycle in the S-phase and induces apoptosis in normal and tumor cell lines, but can be modulated by intracellular levels of cAMP and other factors {Bermejo, L et al (1998) J. Cell Sci 111:637-644;Solovyyan, V et al. (1998) Brain Res Mol Brain Res 62:43-55}

Application

μ For small volumes diluting the stocks 1:10 into fresh DMSO, then diluting the material further 1:100 into the culture media or PBS can facilitate the addition of the inhibitor to small volumes of less than 1 mL.

Domaine de recherche
Apoptose et cancer

Physical form

Liquid in DMSO containing no preservatives.

Preparation Note

° Éviter les congélation/décongélation répétées. Protect product tubes from light.



AVERTISSEMENT : Kit contains highly toxic agents; professional users only; wear gloves and other protective equipment and good laboratory practices when manipulating the concentrated and diluted solutions.

Other Notes

· μ

·

·

·

· 100 μl

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Informations sur la sécurité

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H301,H340,H350,H360FD,H412
• pcodes: P202 - P264 - P273 - P280 - P301 + P310 - P405
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Chronic 3 - Carc. 1B - Muta. 1B - Repr. 1B
• Classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• flash_point_f: 188.6 °F
• flash_point_c: 87 °C