ABC1414

Anti-phospho IGPR-1 (Ser220)

• Synonyme(s) : Transmembrane and immunoglobulin domain-containing protein 2;CD28 homolog;Immunoglobulin and proline-rich receptor 1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Poids mol.: calculated mol wt 30.68 kDa; observed mol wt ~45 to 55 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey
• Conditionnement: antibody small pack of 100 μg
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: C-terminal half
• Numéro d'accès Protein ID: NP_001162597.1
• Numéro d'accès UniProt: Q96BF3
• Température de stockage: -10 to -25°C
• Modification post-traductionnelle de la cible: phosphorylation (pSer220)
• Informations sur le gène: human ... TMIGD2(126259)

Description

Description générale

Transmembrane and immunoglobulin domain-containing protein 2 (UniProt: Q96BF3; also known as CD28 homolog, Immunoglobulin and proline-rich receptor 1, IGPR-1) is encoded by the TMIGD2 (also known as CD28H, IGPR1, UNQ3059/PRO9879) gene (Gene ID: 126259) in human. IGPR-1 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-150), a transmembrane domain (aa 151-171), and a cytoplasmic domain (aa 172-282). It is widely expressed with higher expression observed in epithelial and endothelial cells, including bronchial epithelial cells of lung, breast glandular and lobular epithelia cells. It plays a role in cell-cell interaction, cell migration, and angiogenesis. IGPR-1 is localized to endothelial adherens junctions, and through trans-homophilic dimerization it regulates endothelial cell-cell adhesion and barrier function. This dimerization stimulates phosphorylation of serine 220, which is required for IGPR-1 to regulate endothelial barrier function and angiogenesis. Through interaction with HHLA2, IGPR-1 is reported to co-stimulate T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an Akt-dependent signaling cascade. IGPR-1 is also phosphorylated on serine 220 by I B kinase β, which leads to its activation. Activated IGPR-1 then stimulates phosphorylation of AMPK, which in turn phosphorylates ULK1 and Beclin-1 and leads to increased levels of LC3-II. Genotoxic agents such as doxorubicin and flow shear stress are also reported to simulate phosphorylation of IGPR-1 at serine 220. (Ref.: Amraei, R., et al. (2020). J. Biol. Chem. 295(49); 16691-16699; Wang, YHW., et al.(2016). J. Mol. Biol. 428(24; PtB); 5019-5033).

Spécificité

This rabbit polyclonal antibody detects Immunoglobulin and proline-rich receptor 1 (IGPR-1) phosphorylated on serine 220.

Immunogène

KLH-conjugated linear peptide corresponding to 20 amino acids surrounding phosphoserine 220 in the cytoplasmic domain in Human Immunoglobulin and proline-rich receptor 1 (IGPR-1).

Application

Qualtiy Control Testing

Evaluated by Western Blotting in lysate from HEK293 cells overexpressing IGPR-1.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected IGPR-1 phosphorylated on serine 220 in lysates from HEK293 cells overexpressing IGPR-1.

Tested applications

Immunofluorescence Analysis: A representative lot detected phospho-IGPR-1 (Ser220) in Immunofluorescence applications (Wang, Y., et. al. (2016). J Mol Biol. 428(24 Pt B):5019-5033).

Immunohistochemistry Applications: A representative lot detected phospho-IGPR-1 (Ser220) in Immunohistochemistry applications (Wang, Y., et. al. (2016). J Mol Biol. 428(24 Pt B):5019-5033).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Forme physique

Purified rabbit polyclonal antibody in PBS without azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable