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Principaux documents

AB5330

Sigma-Aldrich

Anti-Syntaxin 4 Antibody

Chemicon®, from rabbit

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

rat

Fabricant/nom de marque

Chemicon®

Technique(s)

western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... STX4(6810)

Spécificité

Recognizes Syntaxin 4.

Immunogène

Highly purified corresponding to residues 2-23 of rat or mouse Syntaxin 4 (Accession Q08850).

Application

Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
This Anti-Syntaxin 4 Antibody is validated for use in WB for the detection of Syntaxin 4.
Western blotting: 0.5-1.0 μg/mL (1:200-1:500) Dilutions should be made using a carrier protein such as BSA (1-3%).


Optimal working dilutions must be determined by the end user.

Forme physique

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.025% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Stockage et stabilité

Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Remarque sur l'analyse

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Mentions de danger

Conseils de prudence

Classification des risques

Aquatic Chronic 3

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Brendan J O'Brien et al.
Molecular vision, 16, 1854-1863 (2010-10-30)
The retina has the demanding task of encoding all aspects of the visual scene within the space of one fixation period lasting only a few hundred milliseconds. To accomplish this feat, information is encoded in specialized parallel channels and passed
Arlene A Hirano et al.
Visual neuroscience, 24(4), 489-502 (2007-07-21)
Horizontal cells mediate inhibitory feed-forward and feedback communication in the outer retina; however, mechanisms that underlie transmitter release from mammalian horizontal cells are poorly understood. Toward determining whether the molecular machinery for exocytosis is present in horizontal cells, we investigated
Brian S Clark et al.
Development (Cambridge, England), 139(9), 1599-1610 (2012-04-12)
To gain insights into the cellular mechanisms of neurogenesis, we analyzed retinal neuroepithelia deficient for Llgl1, a protein implicated in apicobasal cell polarity, asymmetric cell division, cell shape and cell cycle exit. We found that vertebrate retinal neuroepithelia deficient for
Christian Puller et al.
Journal of the Optical Society of America. A, Optics, image science, and vision, 31(4), A189-A194 (2014-04-04)
The distribution of the soluble NSF-attachment protein receptor protein syntaxin-4 and the Na-K-Cl cotransporter (NKCC) were investigated in the outer plexiform layer of human retina using immunohistochemistry. Both proteins, which are proposed to be components of a gamma-aminobutyric acid mediated
Helen Lee et al.
The European journal of neuroscience, 31(8), 1388-1401 (2010-04-14)
Horizontal cells are lateral interneurons that participate in visual processing in the outer retina but the cellular mechanisms underlying transmitter release from these cells are not fully understood. In non-mammalian horizontal cells, GABA release has been shown to occur by

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