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Sigma-Aldrich

Overnight Express™ Instant TB Medium - Novagen

Synonyme(s) :

autoinduction media 

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About This Item

Code UNSPSC :
41106213
Nomenclature NACRES :
NA.54

Forme

solid

Niveau de qualité

200

Fabricant/nom de marque

Novagen®

Conditions de stockage

OK to freeze

Technique(s)

microbiological culture: suitable

Application(s)

microbiology

Conditions d'expédition

ambient

Température de stockage

10-30°C

Description générale

Overnight Express™ Instant TB Medium is a complete autoinduction media in granulated terrific broth formulation. It is used in routine recombinant protein expression applications. Overnight Express Instant TB Medium is ideal for producing recombinant protein without monitoring cell growth or inducer (Isopropyl ß-D-1-thiogalactopyranoside(IPTG)) addition through lac operon-controlled Escherichia coli expression systems.

Application

Overnight Express Instant TB Medium – Novagen has been used:
  • to culture recombinant Escherichia coli colonies for protein expression.
  • as an auto-induction media to study the effect of glucose and lactose additives on recombinant ovine angiotensinogen (oANG) production
  • as a medium for hamster recombinant PrP (rPrP) protein expression

Caractéristiques et avantages

  • Autoinduction media: simplifies protocol by eliminating the monitoring and induction steps
  • Rich culture media: high cell densities and protein expression level
  • Granulated format: rapid and uniform dissolution in water minimize the chance of inhaling hazardous powdered ingredients
  • Singe use EasyPak packaging: Minimal sample handling significantly reduces the chance of contamination.
  • Convenient, no need to spend extra time on weighting out different ingredients

Avertissement

Toxicity: Standard Handling (A)

Notes préparatoires

The EasyPak aluminum foil pouch contains enough granulated medium to prepare 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and bring it to a boil in a microwave oven for 2 minutes.

Informations légales

Express is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Listes réglementaires

Les listes réglementaires sont principalement fournies pour les produits chimiques. Seules des informations limitées peuvent être fournies ici pour les produits non chimiques. L'absence d'indication signifie qu'aucun des composants n'est répertorié. Il incombe à l'utilisateur de s'assurer de l'utilisation sûre et légale du produit.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificats d'analyse (COA)

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Jun-Jie Liu et al.
Journal of immunoassay & immunochemistry, 33(4), 352-368 (2012-09-12)
RGD peptide (Arg-Gly-Asp tripeptide) binds to integrin αVβ(3) and αVβ(5), which is selectively expressed in tumor neovasculature and on the surface of some tumor cells. Some studies showed that coupling the RGD peptides to anticancer drugs yielded compounds with increased
Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 Angstrom resolution.
Mancini, et al.
Protein Science, 16, 2294-2300 (2018)
Nariyasu Tahara et al.
Protein and peptide letters, 28(10), 1180-1190 (2021-08-07)
Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems. Auto-induction can occur unintentionally using a complex culture medium prepared by mixing culture substrates. The differences in culture substrates sometimes lead
Kang Xiao et al.
International journal of molecular medicine, 42(6), 3231-3237 (2018-09-18)
In order to definitively diagnosis sporadic Creutzfeldt‑Jakob disease (sCJD), brain tissue is currently required. Therefore, there is a great need for tests that can detect sCJD in body fluids or other types of tissues. Different variables, including the amount of
Sarah Weisman et al.
Biomaterials, 31(9), 2695-2700 (2009-12-29)
Transgenic production of silkworm and spider silks as biomaterials has posed intrinsic problems due to the large size and repetitive nature of the silk proteins. In contrast the silk of honeybees (Apis mellifera) is composed of a family of four

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