70777
pET Expression System 28 - Novagen
pET vectors plus host strains with induction control
Synonyme(s) :
pET-28a-c(+) Vectors, pET-28a-c(+) vector DNA
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About This Item
Produits recommandés
Étiquette/Marqueur
His tagged
T7 tagged
Niveau de qualité
Fabricant/nom de marque
Novagen®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Conditions d'expédition
dry ice
wet ice
Température de stockage
−70°C
Catégories apparentées
Description générale
pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.
Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.
pET Expression System 28
The pET Expression System 28 contains 10 µg each of the four versions of pET-28 (pET-28a–c(+)). The pET-28a–c(+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB074VM.
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.
Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
- 10 µg pET vector DNA (for each indicated plasmid)
- 0.2 ml BL21 Glycerol Stock
- 0.2 ml BL21(DE3) Glycerol Stock
- 0.2 ml BL21(DE3)pLysS Glycerol Stock
- 0.2 ml Induction Control Glycerol Stock
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
- 0.2 ml NovaBlue Competent Cells
- 0.2 ml BL21(DE3) Competent Cells
- 0.2 ml BL21(DE3)pLysS Competent Cells
- 2 × 0.2 ml SOC Medium
- 10 µl Test Plasmid
Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.
pET Expression System 28
The pET Expression System 28 contains 10 µg each of the four versions of pET-28 (pET-28a–c(+)). The pET-28a–c(+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB074VM.
Composants
- 10 µg- eachpET-28a-c(+) vector DNA
- 0.2 ml- Host bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
- 0.2 ml- Induction control clone, glycerol stock
Avertissement
Toxicity: Standard Handling
Informations légales
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Clickhere for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Clickhere for Enduser Declaration (EUD) Form.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
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