613548

TMB, Insoluble

• Synonyme(s) : TMB, Insoluble, 3,3ʹ,5,5ʹ-Tetramethylbenzidine
• Numéro CAS: 54827-17-7
• Numéro MDL: MFCD00007748
• Code UNSPSC : 41116133
• Nomenclature NACRES : NA.41

Propriétés

• Forme: liquid
• Niveau de qualité: 100
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: clearlight brown
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• Clé InChI: UAIUNKRWKOVEES-UHFFFAOYSA-N

Description

Description générale

Insoluble TMB substrate supplied as a single component system. TMB is the most sensitive chromogenic substrate for detection of horseradish peroxidase (HRP)-labeled probes. In the presence of HRP and hydrogen peroxide (H₂O₂), TMB is oxidized first to a blue, cation free radical; upon further reaction with HRP/H₂O₂, or addition of acid, the radical is converted to a yellow diimine terminal oxidation product. TMB is used routinely in ELISA procedures, whereby its soluble oxidation products are measured spectrophotometrically. For TMB to be worthwhile as a blotting reagent, enhancers such as dextran sulfate or other sugar polymers are usually added.This TMB reagent is a safe, single component system that has been developed for use in blotting procedures by a proprietary methodology that eliminates the use of sugar polymers. The reagent produces a stable, insoluble aquamarine precipitate at the reaction site with little or no background. It can significantly increase the detection limits of assays on a variety of membranes. Ideal for many immunoblotting procedures and can significantly increase detection limits of assays on a variety of membranes

Peroxidase substrate that produces a stable, insoluble, aquamarine blue precipitate at reaction site with little or no background.

Avertissement

Toxicity: Carcinogenic / Teratogenic (D)

Forme physique

1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.

Reconstitution

Discard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H302,H351,H413
• Conseils de prudence: P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313
• Classification des risques: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable