537060

Propidium Iodide Solution

A convenient form of propidium iodide useful for flow cytometry studies.

• Synonyme(s) : Propidium Iodide Solution
• Formule empirique (notation de Hill): C₂₇H₃₄I₂N₄
• Numéro CAS: 25535-16-4
• Poids moléculaire : 668.39
• Numéro MDL: MFCD00011921
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.47

Propriétés

• Niveau de qualité: 100
• Forme: liquid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Entrée: sample type intact cells
• Fluorescence: λ_ex 536 nm; λ_em 617 nm
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• InChI: 1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
• Clé InChI: XJMOSONTPMZWPB-UHFFFAOYSA-M

Description

Description générale

Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Membrane impermeable DNA intercalator

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Forme physique

50 µg/ml in PBS, pH 7.4.

Reconstitution

Following initial use, aliquot and freeze (-20°C).

Autres remarques

Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable