Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

5.31619

Millipore

2-Aminopurine

Synonyme(s) :

2-Aminopurine, 2-AP, 2AP

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Formule empirique (notation de Hill):
C5H5N5
Numéro CAS:
Poids moléculaire :
135.13
Numéro MDL:
Code UNSPSC :
12352200

Essai

≥97% (HPLC)

Niveau de qualité

Forme

powder

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
protect from light

Couleur

yellow

Solubilité

DMSO: 50 mg/mL

Fluorescence

λex 303 nm
λem 370 nm

Température de stockage

2-8°C

InChI

1S/C5H5N5/c6-5-7-1-3-4(10-5)9-2-8-3/h1-2H,(H3,6,7,8,9,10)

Clé InChI

MWBWWFOAEOYUST-UHFFFAOYSA-N

Description générale

A cell permeable adenine analog that is widely used as a versatile fluorescent probe to investigate RNA and DNA secondary and tertiary structures and their conformation dynamics in response to local environment when interacting with other biomolecules with great sensitivity. Forms stable base pairs with neucleobase uracil found in RNA and thymine in DNA, and moderately stable base pairs with cytosine. Exhibits intensive fluorescence emission (lAbsmax = 303 nM, lflmax = 370 nM, and fluorescence quantum yield in solution QFI = 0.68) and longer excitation wavelength compared with other neucleobases enabling its selective excitation with low background signals.
A cell permeable adenine analog that is widely used as a versatile fluorescent probe to investigate RNA and DNA secondary and tertiary structures and their conformation dynamics in response to local environment when interacting with other biomolecules with great sensitivity. Forms stable base pairs with neucleobase uracil found in RNA and thymine in DNA, and moderately stable base pairs with cytosine. Exhibits intensive fluorescence emission (lAbsmax = 303 nM, lflmax = 370 nM, and fluorescence quantum yield in solution QFI = 0.68) and longer excitation wavelength compared with other neucleobases enabling its selective excitation with low background signals.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Actions biochimiques/physiologiques

Cell permeable: yes
Primary Target
DNA and RNA
Reversible: yes

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Ma, L., et al. 2014. Biochem. Biophys. Res. Comm.449, 120.
Widom, J. R., et al. 2013. New. J. Phys.15, 025028.
Souliere, M. F., et al. 2011. J. Am. Chem. Soc.133, 16161,
Jean, J. M., et al. 2001. PNAS98,37.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Acute Tox. 4 Oral

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

J M Jean et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(1), 37-41 (2000-12-20)
2-Aminopurine (2AP) is a fluorescent analog of guanosine and adenosine and has been used to probe nucleic acid structure and dynamics. Its spectral features in nucleic acids have been interpreted phenomenologically, in the absence of a rigorous electronic description of
Long Ma et al.
Biochemical and biophysical research communications, 449(1), 120-125 (2014-05-13)
EcoP15I is a Type III DNA restriction and modification enzyme of Escherichia coli. We show that it contains two modification (Mod) subunits for sequence-specific methylation of DNA and one copy of a restriction endonuclease (Res) subunit for cleavage of DNA
Julia R Widom et al.
New journal of physics, 15 (2013-11-14)
We have observed the conformation-dependent electronic coupling between the monomeric subunits of a dinucleotide of 2-aminopurine (2-AP), a fluorescent analog of the nucleic acid base adenine. This was accomplished by extending two-dimensional fluorescence spectroscopy (2D FS) - a fluorescence-detected variation
Marie F Soulière et al.
Journal of the American Chemical Society, 133(40), 16161-16167 (2011-09-03)
A precise tertiary structure must be adopted to allow the function of many RNAs in cells. Accordingly, increasing resources have been devoted to the elucidation of RNA structures and the folding of RNAs. 2-Aminopurine (2AP), a fluorescent nucleobase analogue, can

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique