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40-014

Millipore

FLEXMAP 3D® System

The FLEXMAP 3D system makes it possible to multiplex up to 500 analytes through the combination of differentially dyed fluorescent microsphere sets & an innovative instrument design.

Synonyme(s) :

3D System, FLEXMAP 3D

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About This Item

Code UNSPSC :
41116133
eCl@ss :
32161000
Nomenclature NACRES :
NB.51

Niveau de qualité

200

Fabricant/nom de marque

Milliplex®

Technique(s)

multiplexing: suitable

Méthode de détection

fluorometric

Description générale

The FLEXMAP 3D® system makes it possible to multiplex up to 500 analytes through the combination of differentially dyed fluorescent microsphere sets and an innovative instrument design. Features such as automated probe height adjustment, simplified routine maintenance operations and an intuitive software interface make FLEXMAP 3D® a flexible and user-friendly multiplexing platform capable of performing a variety of protein and nucleic acid applications. The new xPONENT 4.0 software controls the FLEXMAP 3D system and offers interfacing options for laboratory information systems (LIS) as well as automation.

Application

The FLEXMAP 3D system makes it possible to multiplex up to 500 analytes through the combination of differentially dyed fluorescent microsphere sets & an innovative instrument design.

Informations légales

FLEXMAP 3D is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

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Questions

1–2 of 2 Questions  

  1. I am planning to create a new xPonent protocol template for my assay using a Cytokine Milliplex kit. Which analysis settings do you recommend: Fit of all standards or Mean of replicates?

    1 answer

    1. We generally use the Mean of Replicates method, although Luminex prefers the Fit of All Standards approach.

      Ultimately, the choice between the two methods is left to the researcher to determine the best option for their SOP. Importantly, this choice does not significantly impact sample results.

      Here's a brief overview of the two methods:
      Fit of all standards: This method determines the standard curve by using each individual standard replicate when calculating the standard curve. For instance, if duplicates of a 7-point standard curve are run, the software will calculate the standard curve using 14 points. This method applies only to quantitative analysis.
      Mean of replicates: This method involves averaging the individual standard replicates when calculating the standard curve. Similarly, this method applies only to quantitative analysis. For instance, if duplicates of a 7-point standard curve are run, the software will calculate the standard curve using 7 averaged points.

      Helpful?

  2. Could you provide details on which wells to use on the FM3D probe height adjustment tool for the 384 and 96-well plates utilized in the Milliplex kits?

    1 answer

    1. To use the 96 well plate, utilize well F12 on the probe height adjustment tool (CN-0298-01). For the 384 well mag plate, which is used in the Human High Sensitivity 384 well kit, use well B7.

      Helpful?

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