386032
Anti-Hsp70 Mouse mAb (C92F3A-5)
liquid, clone C92F3A-5, Calbiochem®
Synonyme(s) :
Anti-Heat Shock Protein 70
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
C92F3A-5, monoclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Réactivité de l'espèce (prédite par homologie)
all
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Isotype
IgG1
Conditions d'expédition
wet ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... HSPA1A(3303)
Description générale
Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).
Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.
Immunogène
Hsp70 from HeLa cells
Human
Application
ELISA (see comments)
Flow Cytometry (see comments)
Frozen Sections (5 µg/ml)
Immunoblotting (1 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 µg/ml)
Flow Cytometry (see comments)
Frozen Sections (5 µg/ml)
Immunoblotting (1 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 µg/ml)
Conditionnement
Please refer to vial label for lot-specific concentration.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In PBS, 50% glycerol, pH 7.2.
Reconstitution
Following iniital thaw, aliquot and freeze (-20°C).
Remarque sur l'analyse
Positive Control
L929 cells, Human colon cancer tissue
L929 cells, Human colon cancer tissue
Autres remarques
Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Hang, H., and Fox, M.H. 1995. Cytometry19, 119.
Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.
Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.
Gower, D.J., et al. 1989. J. Neurosurg.70, 605.
Milarski, K., et al. 1989.J. Cell Biol.108, 413.
Vass, K., et al. 1988. Acta Neuropathologica77, 128.
Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.
Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.
Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.
Gower, D.J., et al. 1989. J. Neurosurg.70, 605.
Milarski, K., et al. 1989.J. Cell Biol.108, 413.
Vass, K., et al. 1988. Acta Neuropathologica77, 128.
Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Aigang Lu et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 22(2), 183-195 (2002-02-02)
Estradiol reduces brain injury from many diseases, including stroke and trauma. To investigate the molecular mechanisms of this protection, the effects of 17-beta-estradiol on heat shock protein (HSP) expression were studied in normal male and female rats and in male
Anne K Voss et al.
The EMBO journal, 25(15), 3652-3663 (2006-07-22)
The mechanisms regulating the size of the cerebral cortex are poorly understood. Here, we demonstrate that the Rap1 guanine nucleotide exchange factor, C3G (Grf2, Rapgef1), controls the size of the cerebral precursor population. Mice lacking C3G show overproliferation of the
Daniel Laubitz et al.
Experimental physiology, 91(5), 867-875 (2006-05-27)
The heat shock response is associated with the intracellular expression of a number of highly conserved heat shock proteins (Hsps). According to their molecular size, Hsps have been divided into several groups, which are strongly conserved and show high homology
James H Ahn et al.
Assay and drug development technologies, 9(3), 236-246 (2010-12-08)
Heat shock protein 70 (Hsp70) is a chaperone protein that helps protect against cellular stress, a function that may be co-opted to fight human diseases. In particular, the upregulation of Hsp70 can suppress the neurotoxicity of misfolded proteins, suggesting possible
Rosario Barranco et al.
International journal of legal medicine, 133(5), 1461-1467 (2019-06-22)
The diagnosis of drowning is one of the most difficult in forensic medicine. The aim of this study was to analyze pulmonary tissue reactions in death by drowning. In particular, we focused on the immunohistochemical expression of P-selectin, SP-A, HSP70
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