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Merck
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Principaux documents

126593

Sigma-Aldrich

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade

Synonyme(s) :

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.25

Qualité

RIA grade

Pureté

≥98% (agarose gel electrophoresis)

Forme

solid

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Impuretés

≤3.0 ash
≤5.0% moisture

Couleur

off-white

Solubilité

0.15 M sodium chloride: 10 mg/mL

Traces de cations

heavy metals: ≤10 ppm

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade is a purified plasma protein that is useful as a stabilizing agent and as a carrier protein. It is prepared under non-denaturing conditions by modification of the Cohn procedure and caprylic acid heat-shock treatment.
Bovine serum albumin (BSA) is an α-helical, non-glycosylated globular protein with 17-disulfide bonds.

Application

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade has been used:
  • to block tissue sections of placenta in immunohistochemical (IHC) analysis
  • as a component of Krebs buffer for in vitro glucose-stimulated insulin secretion (GSIS) experiments and drug/palmitate preparation
  • as a vehicle control to incubate whole mouse blood to study plate aggregation in vivo

Actions biochimiques/physiologiques

Bovine Serum Albumin (BSA), an important component in the cell culture media can transport fatty acids, hormones, and drugs. It is generally used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Jessica Suagee-Bedore et al.
Animals : an open access journal from MDPI, 11(12) (2021-12-25)
Older horses and those prone to obesity may be at a higher risk for inflammation than younger and leaner counterparts. Previous research indicated a postprandial elevation in plasma concentrations of interleukin-1β (IL-1β), a pro-inflammatory cytokine, after consuming 1.2 g of
Ernest T Parker et al.
Bio-protocol, 11(20), e4195-e4195 (2021-11-12)
Diffusion is a fundamental process in biological systems that governs the molecular collisions driving biochemical reactions and membrane and transport. Measurement of the diffusion coefficient and application of the Stokes-Einstein equation produces the hydrodynamic radius, which is a commonly used
Qi Da et al.
Microcirculation (New York, N.Y. : 1994), 25(6), e12457-e12457 (2018-04-28)
Monitoring endogenous platelets during intravital microscopy often involves two approaches: fluorescently labeled antibodies or genetic models of platelet-specific fluorescent protein expression. Due to limited data available on platelet functional changes induced by these methods, we compared functional effects of these
Katherine Paschalidou et al.
The Biochemical journal, 382(Pt 3), 1031-1038 (2004-07-06)
The development of renin inhibitors for the treatment of hypertension requires highly sensitive substrates to evaluate potency and to characterize the mechanism of tight-binding inhibitors. A series of intramolecularly quenched fluorogenic renin substrates, based on the N-terminal tetradecapeptide sequence of
Lucas A J Ahrens et al.
PloS one, 12(4), e0175095-e0175095 (2017-04-06)
Controlled release drug delivery systems are well established as oral and implantable dosage forms. However, the controlled release paradigm can also be used to present complex soluble signals responsible for cellular organization during development. Endochondral ossification (EO), the developmental process

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