1.25069

Fractogel^® EMD TMAE (M)

MiniChrom Column Fractogel^® TMAE (M), volume 5 mL, prepacked column

• Synonyme(s) : MiniChrom Column Fractogel^® TMAE (M), 5 ml
• Code UNSPSC : 41115711

Propriétés

• ligand: (Trimethylammoniumethyl)
• Matériaux: polypropylene housing
• Gamme de produits: Fractogel^®
• Forme: prepacked column
• Paramètres: ≤8 bar pressure; ~240 cm/hr max. flow rate
• Longueur du lit: 100 mm
• Volume du lit: 5 mL
• D.I. de la colonne: 8 mm
• Volume: 5 mL
• Groupe de la matrice active: Methacrylate
• Taille moyenne des particules: 40-90 μm
• Capacité: ~100 mg, BSA binding capacity
• Application(s): gene therapy; mAb; recombinant protein
• Technique de séparation: anion exchange
• Température de stockage: 15-25°C

Description

Description générale

8 x 100 mm

MiniChrom Pre-packed Columns with Fractogel^® cation exchange resins are designed for the rapid evaluation of resins for bioprocess development. The results obtained for biomolecule separations are generally comparable to the results achieved using laboratory-scale columns (e.g. 100 x 16 mm ID).

In bioprocess design, the MiniChrom columns can be used to rapidly determine whether a target biomolecule is bound to and can be eluted from the Fractogel^® resin, as well as determine whether an appropriate target yield may be achieved and whether major contaminants could potentially be removed.

MiniChrom columns are designed for process development, method optimization and parameter screening as well as sample preparation. MiniChrom columns are quality checked for HETP and asymmetry, providing superior reproducibility, excellent scalability, convenient comparison of different chromatography media.

MiniChrom columns are made from biocompatible polypropylene (PP) and polyethylene (PE) and are available in 1 and 5 mL bed volumes. The 8mm diameter columns allow for scale-up from 1 to 5mL column volumes, with a constant internal diameter. These columns are compatible with any chromatographic work station, HPLC system or peristaltic pump.

Caractéristiques et avantages

MiniChrom Column Fractogel^® TMAE (M), 5 ml enables:

• High protein loading conditions resulting in good selectivity and resolution with the tentacle technology
• Excellent binding to large viruses at increased loading
• Homogenous binding with high selectivity and purity
• Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Informations légales

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany