06-562

Anti-erbB-2/HER-2 Antibody

Upstate^®, from rabbit

• Synonyme(s) : CD340 antigen, CD340 antigen, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, c-erb B2/neu protein, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog),
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: monkey, human, mouse
• Réactivité de l'espèce (prédite par homologie): rat
• Fabricant/nom de marque: Upstate^®
• Technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NM_004448
• Numéro d'accès UniProt: P04626
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ERBB2(2064) ; mouse ... Erbb2(13866) ; rat ... Erbb2(24337) ; rhesus monkey ... Erbb2(697573)

Description

Description générale

The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.

Spécificité

Recognizes c-erbB-2 (185 kDa), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.

Immunogène

A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein.

Application

Anti-erbB-2/HER-2 Antibody is an antibody against erbB-2/HER-2 for use in IH, IP & WB.

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated erbB-2/HER-2 from 600 μg MCF-7 RIPA lysate, and previously in A431, LNCaP, and c-erbB-2 transfected 3T3/NIH RIPA lysate.

Immunohistochemistry:
A previous lot of antibody has been reported by an independent laboratory, to detect erbB-2/HER-2 in frozen and formalin/paraffin sections of rat breast.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Qualité

Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Her-2 on 10 μg of HEK293 lysates.

Description de la cible

185 kDa

Liaison

Replaces: 04-1127

Forme physique

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 10 mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Frozen solution.

Stockage et stabilité

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
A431 cells, HEK293, Jurkat, MCF7 cell lysate.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable