Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

Voir toute la documentation

04-902

Sigma-Aldrich

Anti-HA Tag Antibody, clone DW2, rabbit monoclonal

culture supernatant, clone DW2, from rabbit

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

culture supernatant

Type de produit anticorps

primary antibodies

Clone

DW2, monoclonal

Espèces réactives

vertebrates (common species)

Technique(s)

western blot: suitable

Isotype

IgG

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Description générale

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Spécificité

Reactivity with other species has not been confirmed.
Recognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.

Immunogène

KLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.

Application

Anti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.

Qualité

Western Blot Analysis
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.

Description de la cible

Various

Liaison

Replaces: 05-902

Forme physique

Cultured supernatant in 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
HA-tagged WNT-1 protein.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ivonne Melano et al.
International journal of molecular sciences, 25(1) (2024-01-11)
How ACE2 functions as the major host receptor of SARS-CoV-2 despite having low expression in the lungs is still unknown. To facilitate the development of therapeutic strategies against coronaviruses, gaining a deeper comprehension of the molecular mechanism of SARS-CoV-2 infection
Elinn Johansson et al.
Cell reports, 20(7), 1641-1653 (2017-08-17)
Hypoxia-inducible factors enhance glioma stemness, and glioma stem cells have an amplified hypoxic response despite residing within a perivascular niche. Still, little is known about differential HIF regulation in stem versus bulk glioma cells. We show that the intracellular domain
Ivonne Melano et al.
Viruses, 13(7) (2021-08-11)
Amino acids have been implicated with virus infection and replication. Here, we demonstrate the effects of two basic amino acids, arginine and lysine, and their ester derivatives on infection of two enveloped viruses, SARS-CoV-2, and influenza A virus. We found
Lei Dai et al.
Nature communications, 7, 11996-11996 (2016-06-30)
SARI, also called as BATF2, belongs to the BATF family and has been implicated in cancer cell growth inhibition. However, the role and mechanism of SARI in tumour angiogenesis are elusive. Here we demonstrate that SARI deficiency facilitates AOM/DSS-induced colonic
Abdo J Najy et al.
The Journal of biological chemistry, 283(26), 18393-18401 (2008-04-25)
The zinc-dependent disintegrin metalloproteinases (a disintegrin and metalloproteinases (ADAMs) have been implicated in several disease processes, including human cancer. Previously, we demonstrated that the expression of a catalytically active member of the ADAM family, ADAM15, is associated with the progression
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique