SRP3280

SCD27 LIGAND human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(s): CD70, TNFSF7
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in CHO cells
• Assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• potency: 10.0-25.0 ng/mL
• mol wt: 25.0-35.0 kDa
• packaging: pkg of 50 μg
• impurities: <0.1 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: P32970
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CD70(970)

Description

General description

CD27 Ligand, a type II transmembrane protein, is expressed on activated T and B lymphocytes as well as NK cells. CD27L and its receptor (CD27) regulate the immune response by promoting T-cell expansion and differentiation, as well as NK enhancement. CD27 signaling can act as a co-stimulatory effector to sustain the survival of CD8+ T cells, primarily by inducing increased expression of the IL-2 gene.

Biochem/physiol Actions

CD27 Ligand, a type II transmembrane protein, is a member of the TNF superfamily. Full length human CD27L is a 193 amino acid protein, consisting of a 17 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 155 amino acid extracellular domain. Human soluble CD27L corresponds to the 155 amino acid extracellular domain of the full length CD27L protein. Recombinant human sCD27L contains the extracellular domain plus an N-terminal His-Tag.

Physical form

Lyophilized from 20 mM Sodium Citrate, pH 3.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable