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Key Documents

P0075

Sigma-Aldrich

Anti-PINK1 (N-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-BRPK, Anti-PARK6, Anti-PTEN-induced putative kinase 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

western blot: 4-8 μg/mL using HEK-293T cell lysate expressing human PINK1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PINK1(65018)

General description

PINK1 (PTEN induced putative kinase 1, also known as PARK6, BRPK), has been identified as linked to the autosomal recessive form of familial Parkinson′s disease (PD). PINK1 is a Ser/Thr kinase that has been localized to the mitochondria. PINK1 contains an N-terminal mitochondrial targeting motif and a highly conserved kinase domain homologous to Ser/Thr kinases of the Ca2+/calmodulin family.

Application

Anti-PINK1 (N-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

PINK1 (PTEN induced putative kinase 1) has been found to protect neurons from stress-induced mitochondrial dysfunction and apoptosis. It may protect cells from stress-induced mitochondrial dysfunction. Genetic studies in Drosophila indicate that PINK1 acts upstream of Parkin in a common pathway that influences mitochondrial morphology. PINK1 elicits protection in mouse primary neurons from the dopaminergic neurotoxin 1-methyl-4-phenylpyridine (MPP+)/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) both in vitro and in vivo. In response to enhanced proteasomal stress in vitro, PINK1 has been shown to be cleaved and localized to the mitochondria, and this correlates with increased expression of the processed PINK1 protein in Parkinson′s disease (PD) brain.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Altered cleavage and localization of PINK1 to aggresomes in the presence of proteasomal stress
Muqit MMK, et al.
Journal of Neurochemistry, 98(1), 156-169 (2006)
Hereditary early-onset Parkinson's disease caused by mutations in PINK1
Valente EM, et al.
Science (New York, N.Y.), 304(5674), 1158-1160 (2004)
Cytoplasmic Pink1 activity protects neurons from dopaminergic neurotoxin MPTP
Haque ME, et al.
Proceedings of the National Academy of Sciences of the USA, 105(5), 1716-1721 (2008)
Mitochondrial dysfunction in Drosophila PINK1 mutants is complemented by parkin
Park J, et al.
Nature, 441(7097), 1157-1157 (2006)
Cristina Guardia-Laguarta et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(36), 7074-7085 (2019-07-14)
Maintaining a pool of functional mitochondria requires degradation of damaged ones within the cell. PINK1 is critical in this quality-control process: loss of mitochondrial membrane potential causes PINK1 to accumulate on the mitochondrial surface, triggering mitophagy. However, little is known

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