H8651

HEPES sodium salt

BioXtra, ≥99.0% (titration)

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
• Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₄S
• CAS Number: 75277-39-3
• Molecular Weight: 260.29
• EC Number: 278-169-7
• MDL number: MFCD00036463
• UNSPSC Code: 12161700
• PubChem Substance ID: 24895527
• NACRES: NA.25

Properties

• product line: BioXtra
• Assay: ≥99.0% (titration)
• form: powder
• impurities: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• pH: 10.0-12.0 (1 M in H₂O)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: H₂O: 1 M, clear, colorless
• anion traces: chloride (Cl^-): ≤0.05%; sulfate (SO₄^2-): ≤0.05%
• cation traces: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Pb: ≤0.001%; Zn: ≤0.0005%
• absorption: ≤0.2 at 260 in H₂O at 1 M; ≤0.2 at 280 in H₂O at 1 M
• application(s): diagnostic assay manufacturing
• SMILES string: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• InChI key: RDZTWEVXRGYCFV-UHFFFAOYSA-M

Description

General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Other Notes

Easily compare specifications for HEPES sodium salt products with the HEPES sodium salt specification table.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves