G8885
β-Glucuronidase from Helix pomatia
Type H-3, aqueous solution, ≥90,000 units/mL
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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All Photos(3)
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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type
Type H-3
form
aqueous solution
specific activity
≥90,000 units/mL
secondary activity
≤1,000 units/mL sulfatase
shipped in
wet ice
storage temp.
2-8°C
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General description
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Application
β-Glucuronidase from Helix pomatia has been used:
- to convert isoflavone conjugates to their aglycone forms in urine and soymilk samples for high performance liquid chromatography (HPLC) detection
- as a component in the protoplasting solution
- for the generation of aromatic and phenolic compounds from the plasma and urine samples for gas chromatography-mass spectrometry (GC-MS)
- to hydrolyze glucuronide and sulfate conjugated metabolites in fecal samples
β-glucuronidase was used in the measurement of aromatization by a urine technique suitable for the evaluation of aromatase inhibitors in vivo.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol Actions
β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Quality
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Physical form
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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