E0276

EPPS

BioPerformance Certified, suitable for cell culture, ≥99.5% (titration)

• Synonym(s): 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), HEPPS
• Empirical Formula (Hill Notation): C₉H₂₀N₂O₄S
• CAS Number: 16052-06-5
• Molecular Weight: 252.33
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 24894366
• EC Number: 240-198-8
• Beilstein/REAXYS Number: 3957385
• MDL number: MFCD00006160

Properties

• grade: BioPerformance Certified
• Quality Segment: 400
• assay: ≥99.5% (titration)
• form: crystalline powder
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin and Total Aerobic Microbial Count, tested
• pH: 7.3-8.7
• useful pH range: 7.3-8.7
• pKa (25 °C): 8.0
• pKa: 8
• mp: 237-239 °C (lit.)
• solubility: H₂O: 25 g + 50 mL, clear, colorless
• cation traces: heavy metals (as Pb): ≤5 ppm
• λmax: A290 at ≤0.1 (C=33% w/w)
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCCS(O)(=O)=O)CC1
• InChI: 1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)
• InChI key: OWXMKDGYPWMGEB-UHFFFAOYSA-N

Description

General description

EPPS or 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (HEPPS) is a zwitterionic buffer substance from the group of good buffers. It effectively maintains constant pH in a solution, which is necessary for many biological and biochemical reactions. EPPS buffer is widely used in cell culture experiments and biological research.

Application

EPPS may be used as a buffer solution to stabilize the pH in sea water samples during the titration experiments.

Features and Benefits

• BioPerformance Certified, high-quality level (400), ensures reliable and consistent results.
• Suitable for mammalian cell culture techniques

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)