For an MTT assay, standard RPMI or DMEM media are suitable options. These include RPMI (Product R0883) and DMEM (Product D5671), both of which may contain up to 10% FBS. When performing assays, such as mitogen or complement-mediated cytotoxicity assays, cells should be suspended in a tissue culture medium, such as DMEM or RPMI, before adding the MTT reagent to each well.
D5030
Dulbecco′s Modified Eagle′s Medium
Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DME, DMEM
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Pricing and availability is not currently available.
Quality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.
Application
Dulbecco′s Modified Eagle′s Medium has been used:
- to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
- to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
- to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells
Preparation Note
Formulated to contain 8.3 grams of powder per liter of medium.
Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.
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This Item | |||
|---|---|---|---|
| form powder | form liquid | form powder | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components phenol red: no | components phenol red: no | components L-glutamine: yes | components phenol red: yes |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 500 | Quality Level 400 | Quality Level 500 | Quality Level 500 |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Is there an available variant of EMEM that is free from phenol red, glutamine, and NaHCO3? Additionally, for an MTT assay, would it be necessary to have two types of media – one being the aforementioned EMEM variant and the other for regular culture growth?
1 answer-
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Is the CaCl2 included in Product D5030 in the form of a dihydrate or is it anhydrous?
1 answer-
The formulation utilizes Calcium Chloride Anhydrous rather than the dihydrate form.
Helpful?
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What are the preparation instructions of the 10L product?
1 answer-
As listed on the product detail page in the 'DESCRIPTION' section under 'Reconstitution', this product can be supplemented with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.
Helpful?
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How can we reconstitute this powder to create ready-to-use media? What is the reason for excluding Sodium bicarbonate from powdered media?
How can we reconstitute this powder to create ready-to-use media? What is the reason for excluding Sodium bicarbonate from powdered media?
0 answers