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C5275

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV-S, lyophilized powder, aseptically processed, BioReagent, suitable for cell culture

Synonym(s):

Con A

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
23151817
NACRES:
NA.32

sterility

aseptically processed

Quality Level

type

Type IV-S

product line

BioReagent

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

impurities

salt (Contains buffer salts and NaCl)

solubility

PBS: 5 mg/mL, slightly hazy
H2O: soluble, slightly hazy

storage temp.

−20°C

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General description

Concanavalin A is a protein containing only β-sheets in its structure, and belongs to the legume lectins family.

Application

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
  • in in vitro cell proliferation assay and cell cycle assessment
  • in lymphocyte proliferation
  • in splenocyte culturing

Biochem/physiol Actions

Concanavalin A is known to stimulate acute hepatic inflammation and acts as a T‐cell mitogen. It has inhibitory effect on hepatocellular carcinoma by inducing autophagy.
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Analysis Note

A lymphocyte transformation assay is used to determine suitability for cell culture applications. The mitogenic activity of this product is assessed by bromodeoxyuridine incorporation and is found to have peak incorporation at ≤75 ug lectin/ml of medium.
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

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Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Evaluation of immune functions in captive immature loggerhead sea turtles (Caretta caretta)
Rousselet E, et al.
Veterinary Immunology and Immunopathology, 156(1-2), 43-53 (2013)
Effect of Tapentadol on Splenic Cytokine Production in Mice
Franchi S, et al.
Anesthesia and Analgesia, 124(3), 986-995 (2017)
Amyloid fibrils formation and amorphous aggregation in concanavalin A
Vetri V, et al.
Biophysical Chemistry, 125(1), 184-190 (2007)
Effect of Varying Proportions of Lignin and Cellulose Supplements on Immune Function and Lymphoid Organs of Layer Poultry (Gallus gallus)
Hussein SM and Frankel TL
The journal of poultry science, 0180032-0180032 (2018)
Sean J Judge et al.
The Journal of clinical investigation, 130(6), 3051-3068 (2020-03-07)
PD-1 expression is a hallmark of both early antigen-specific T cell activation and later chronic stimulation, suggesting key roles in both naive T cell priming and memory T cell responses. Although significant similarities exist between T cells and NK cells

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