A8850

Auto-Activated Protein Kinase from bovine kidney

from bovine kidney, aqueous solution

• Synonym(s): AK
• UNSPSC Code: 12352204
• NACRES: NA.32
• MDL number: MFCD03792079
• Specific activity: ~17,000 units/mg protein
• Biological source: bovine kidney

Properties

• biological source: bovine kidney
• Quality Segment: 200
• description: Minimum 50 ng protein/vial by Bradford
• form: aqueous solution
• specific activity: ~17,000 units/mg protein
• mol wt: 36 kDa
• shipped in: dry ice
• storage temp.: −70°C

Description

Biochem/physiol Actions

This enzyme phosphorylates and inactivates protein phosphatase 2A. The funtion appears to be related to the catalytic domain of p21-activated p65^(PAK) protein kinase which is produced in apoptotic cells. AK is also involved in cytoskeleton organization and other signal transduction processes.

Physical form

50 ng per vial in 10 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-Mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, 65% ethyleneglycol, and 0.1% Brij^® 35.

Other Notes

One unit is the amount of enzyme that will incorporate 1 nmole of phosphoryl groups using myelin basic protein as a substrate per minute at pH 7 at 30 deg C.

Legal Information

Brij is a registered trademark of Croda International PLC

Safety Information

• Storage Class: 10 - Combustible liquids