Skip to Content
Merck
All Photos(1)

Key Documents

A6907

Sigma-Aldrich

Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Human IgM (μ-chain specific)−HRP

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

0.5-4 mg/mL

technique(s)

direct ELISA: 1:10,000

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

General description

Human IgM antibodies are glycoproteins that are mainly produced by the B-1 cells and regulate the physiology and growth of the human B cell reserve. Circulating monoclonal IgM antibody that binds to myelin-associated glycoprotein has been linked to the demyelination of peripheral nerves in humans . Anti-human IgM μ-chain specific−peroxidase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones κ and Lambda myeloma proteins.
There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM) is a high molecular weight protein that has five to six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates.

Immunogen

human IgM

Application

Anti-Human IgM (μ-chain specific)-Peroxidase antibody has been used in direct binding assays to detect antiphospholipid antibodies (aPL).
Anti-human IgM (μ-chain specific)-Peroxidase antibody is suitable for use in direct ELISA (1:10,000 and 1:5000). It has also been used in indirect ELISA (1:2000). The concentration of IgM in supernatant samples was determined by ELISA using HRP-conjugated goat anti-human chain specific IgG as the secondary antibody. The antibody has also been used in Western blotting.

Biochem/physiol Actions

Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% BSA with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jiajun Zhou et al.
Toxicological sciences : an official journal of the Society of Toxicology, 163(1), 319-329 (2018-02-21)
Aryl hydrocarbon receptor (AHR) activation by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) is well established at suppressing humoral immunity. Previous studies in mouse B cells revealed that decreased IgM production was due to a significant suppression in the mRNA levels of
Measuring IgA anti-beta2-glycoprotein I and IgG/IgA anti-domain I antibodies adds value to current serological assays for the antiphospholipid syndrome
Pericleous C, et al.
PLoS ONE, 11(6), e0156407-e0156407 (2016)
Yun Zhou et al.
Hepatology communications, 3(2), 293-304 (2019-02-16)
Bacterial infection is frequently observed in patients with alcoholic liver disease (ALD). We examined a possible role of Porphyromonas gingivalis in the development/progression and severity of disease in patients with acute alcoholic hepatitis (AAH). Plasma specimens from 47 patients with
Production of IgM Hexamers by Normal and Autoimmune B Cells: Implications for the Physiologic Role of Hexameric IgM
Hughey, C
Journal of Immunology, 8, 4091-4097 (1998)
Suzanne Pickering et al.
PLoS pathogens, 16(9), e1008817-e1008817 (2020-09-25)
There is a clear requirement for an accurate SARS-CoV-2 antibody test, both as a complement to existing diagnostic capabilities and for determining community seroprevalence. We therefore evaluated the performance of a variety of antibody testing technologies and their potential use

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service