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71636

Sodium phosphate dibasic

BioUltra, Molecular Biology, ≥99.5% (T)

Sodium phosphate dibasic

Synonym(s):

sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate

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About This Item

Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
PubChem Substance ID:
eCl@ss:
38070211
UNSPSC Code:
12161700
NACRES:
NA.25
EC Number:
231-448-7
MDL number:
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grade

Molecular Biology

Quality Level

product line

BioUltra

assay

≥99.5% (T)

form

coarse powder, fine crystals, powder

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% total nitrogen (N)

loss

≤0.1% loss on drying, 110 °C

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤10 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.2 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤10 mg/kg, Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.025, λ: 280 nm Amax: ≤0.020

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

Application

Sodium phosphate dibasic has been used:
  • as a component in phosphate buffer
  • in the preparation of MgCl2 hybridization buffer
  • as a component in SSPE solution for the preparation of small RNA hybridisation buffer[1]


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This Item
S3264S790771642
grade

Molecular Biology

grade

Molecular Biology

grade

-

grade

-

assay

≥99.5% (T)

assay

≥98.5% (titration)

assay

≥99.0%

assay

≥98.0% (T)

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

300

product line

BioUltra

product line

-

product line

BioXtra

product line

-

form

coarse powder, fine crystals

form

powder

form

powder or crystals

form

powder

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

H2O: 100 mg/mL

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

water: soluble 50 mg/mL at 25 °C


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Storage Class

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Foundations of identifying individual chromosomes by imaging flow cytometry with applications in radiation biodosimetry
Beaton-Green LA, et al.
Methods, 112, 18-24 (2017)
Design of biomimetic collagen matrices by reagent-free electron beam induced crosslinking: Structure-property relationships and cellular response
Riedel S, et al.
Materials & Design, 107606-107606 (2019)
Eva Várallyay et al.
Nature protocols, 3(2), 190-196 (2008-02-16)
MicroRNAs (miRNAs) are short, about 21 nucleotides in length, noncoding, regulatory RNA molecules representing a new layer in post-transcriptional regulation of gene expression. Intensive miRNA research has necessitated the development of effective miRNA detection methods such as northern analyses, quantitative



Global Trade Item Number

SKUGTIN
1199002-200MG04061838712356
BP85504061835276127
D180000004061833792902
D5878-250MG04061833586945
PHL82516-25MG04061835495382
D5878-5G04061833629642
D5878-1G04061833586938
D5878-500MG04061833586952
D5878-10G04061833468258
71636-250G04061832849973
71636-1KG04061832849942

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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Reviews

Active Filters

  1. Italy
    • Reviews 3
    • Votes 3
    5 out of 5 stars.

    it's suitable for the preparation of phosphate buffer

    it's suitable for the preparation of phosphate buffer

    Helpful?