CRM13216L

Escherichia coli NCTC 13216 LENTICULE^® discs

30-120 CFU, suitable for microbiology, certified reference material, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

• Synonym(s): E. coli NCTC 13216 Low count Lenticule^® discs, Certified reference material, Microbiological standards
• UNSPSC Code: 41116107
• NACRES: NA.24

Properties

• grade: certified reference material
• Quality Level: 300
• Agency: ISO 17034; ISO/IEC 17025
• form: disc (hydrosoluble); solid
• shelf life: limited shelf life, expiry date on the label
• packaging: pack of 10
• manufacturer/tradename: Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
• application(s): environmental; food and beverages; microbiology
• storage temp.: −20°C
• suitability: Escherichia coli; Escherichia spp.; coliforms

Description

General description

Escherichia coli NCTC 13216 Low count Lenticule^® discs are Certified Microorganisms Standards (Certified Microbiological Reference Materials) which can be used as quantitative and qualitative control organisms. They contain colony forming units (CFUs) in the range of 30-120 CFU per disc. The technology allows long-term conservation and renders the organisms resistant to temperature changes. They are intended to be used as daily internal quality control organisms. These microorganisms are certified according to ISO/IEC 17025 and produced under reproducible conditions compliant with ISO 17034 using authenticated strains from NCTC. These ready-to-use microbiological controls minimize the need to maintain control strains in the test laboratory and guarantee an authenticated control culture for every quality control test. Lenticule^® discs are prepared from a traceable culture obtained freeze-dried from the National Collection of Type Cultures (NCTC) or National Collection of Pathogenic Fungi (NCPF) and are manufactured by Sigma-Aldrich under license from UK Health Security Agency.

Application

Escherichia coli NCTC 13216 Low count Lenticule^® discs are used as reference strains in routine quality control for food, beverage, water, and environmental testing. Additionally, they are excellent for use in method development and validations, as starter cultures, and for research studies and educational purposes.

Features and Benefits

• Ready-to-use at convenient CFU ranges
• No dilution required
• Quick rehydration in 10 minutes
• At least 16 months of shelf life in a standard -20 °C freezer
• Complete with a comprehensive certificate of analysis
• No maintenance of stock cultures
• No recovery time or pre-enrichment step is necessary
• Robust, efficient, and reliable
• Easy-to-use
• Cost-effective

Other Notes

Strain origin: NCTC 13216

Other cell culture collections:

• CECT^® 9153

Click here to access the BacDive database for more info on metabolism, genome, growth media, and more

Strain History - Accessioned into the NCTC in 2001 < S. Brimble (CPHL EQA, Colindale, London, UK) < Isolated by Nicola Gough from unpasteurised brie in UK, 1996.

Legal Information

CECT is a registered trademark of Universitat de Valencia

Lenticule is a registered trademark of The Secretary of State for Health (United Kingdom)

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3