11093657910
Roche
DIG DNA Labeling and Detection Kit
Synonym(s):
dna labeling and detection kit, dig
Sign Into View Organizational & Contract Pricing
All Photos(3)
About This Item
UNSPSC Code:
41105500
Recommended Products
usage
sufficient for 25 labeling reactions
sufficient for 50 blots
Quality Level
manufacturer/tradename
Roche
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Aligned
storage temp.
−20°C
General description
DIG DNA Labeling and Detection Kit is a convenient kit for random-primed labeling of DNA with digoxigenin-deoxyuridine triphosphate (dUTP), alkali-labile and color detection of hybrids by enzyme immunoassay. In this method, the complementary strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of random oligonucleotides as primers.
Contents:
Contents:
- Unlabeled Control DNA 1, 100 μg/ml
- Unlabeled Control DNA 2, 200 μg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA, 5.2 μg/ml
- 10x Hexanucleotide Mix
- 10x dNTP Labeling Mixture
- Klenow Enzyme, Labeling grade, 2 U/μl
- Anti-digoxigenin-AP-conjugate, 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Specificity
Sensitivity and specificity: The standard labeling reaction with 1 μg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.
Application
DIG DNA Labeling and Detection Kit has been used in a variety of hybridization techniques:
- Southern blots
- Northern blots
- Dot blots
- Colony and plaque screening
Packaging
1 kit containing 10 components.
Preparation Note
Working concentration: Dilution of Antibody 1:5,000
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Unlabeled Control DNA 1 100 µg/ml
- Unlabeled Control DNA 2 200 µg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA 5.2 µg/ml
- Hexanucleotide Mix 10x concentrated
- dNTP Labeling Mixture 10x concentrated
- Klenow Enzyme, Labeling grade 2 U/µl
- Anti-digoxigenin-AP-conjugate antibody 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
See All (10)
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
does not flashNot applicable
Flash Point(C)
does not flashNot applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Nasser Shakhssalim et al.
Cancer cell international, 13(1), 120-120 (2013-12-07)
Bladder cancer is a relatively common and potentially life-threatening neoplasm that ranks ninth in terms of worldwide cancer incidence. The aim of this study was to determine deletions and sequence variations in the mitochondrial displacement loop (D-loop) region from the
Hongshun Li et al.
International journal of molecular sciences, 22(11) (2021-06-03)
The vegetative phase transition is a prerequisite for flowering in angiosperm plants. Mulberry miR156 has been confirmed to be a crucial factor in the vegetative phase transition in Arabidopsis thaliana. The over-expression of miR156 in transgenic Populus × canadensis dramatically
Handeng Liu et al.
Journal of invertebrate pathology, 99(2), 235-238 (2008-07-22)
Among Microsporidia, Nosema bombycis has a novel arrangement of LSUrRNA, SSUrRNA, ITS, IGS and 5SrRNA. To determine the distribution of rDNA among the chromosomes, we performed genome-wide screening and Southern blotting with three probes (SSU, ITS and IGS). Southern blotting
Jiaqiang Wang et al.
Cell, 175(7), 1887-1901 (2018-12-15)
In early mammalian embryos, it remains unclear how the first cell fate bias is initially triggered and amplified toward cell fate segregation. Here, we report that a long noncoding RNA, LincGET, is transiently and asymmetrically expressed in the nucleus of
Shun Sawatsubashi et al.
Scientific reports, 8(1), 593-593 (2018-01-14)
CRISPR/Cas9-based genome editing has dramatically accelerated genome engineering. An important aspect of genome engineering is efficient knock-in technology. For improved knock-in efficiency, the non-homologous end joining (NHEJ) repair pathway has been used over the homology-dependent repair pathway, but there remains
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service