MPTMAG-49K
MILLIPLEX® Mouse Pituitary Magnetic Bead Panel - Endocrine Multiplex Assay
The analytes available for this multiplex kit are: ACTH, BDNF, FSH, GH, Prolactin, TSH, LH.
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Recommended Products
Quality Level
species reactivity
mouse
manufacturer/tradename
Milliplex®
assay range
accuracy: 96-101%
standard curve range: 12.2-50,000 pg/mL
(TSH)
standard curve range: 122-500,000 pg/mL
(Prolactin)
standard curve range: 2.4-10,000 pg/mL
(ACTH)
standard curve range: 2.4-10,000 pg/mL
(BDNF)
standard curve range: 24.4-100,000 pg/mL
(FSH)
standard curve range: 4.9-20,000 pg/mL
(GH)
standard curve range: 4.9-20,000 pg/mL
(LH)
inter-assay cv: <20%
intra-assay cv: <15%
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
Called the “master gland” because it controls many other endocrine glands, the pituitary secretes several key hormones that play important roles in the regulation of metabolism, growth, and reproduction. Piuitary hormones are responsible for the stimulation of the adrenal gland (ACTH), thyroid gland (TSH), ovaries and testes (FSH, LH) and breast milk production (prolactin), as well as blood pressure and the amount of water excreted by the kidneys. Through its connection with the pituitary, the hypothalamus modulates endocrine function by detecting hormone levels. Brain-Derived Neurotrophic Factor (BDNF) is a member of NGF family of neurotrophic factors which are required for differentiation and survival of specific neuronal sub-populations.
MILLIPLEX® Mouse Pituitary Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in mouse serum, plasma, and tissue culture samples: ACTH, BDNF, FSH, GH, Prolactin, TSH and LH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
MILLIPLEX® Mouse Pituitary Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in mouse serum, plasma, and tissue culture samples: ACTH, BDNF, FSH, GH, Prolactin, TSH and LH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
Application
- Analytes: ACTH, BDNF, FSH, GH, LH, Prolactin, TSH
- Recommended Sample Type: Mouse serum, plasma, cell/tissue culture supernatants or lysates
- Recommended Sample Dilution: 10 μL per well of undiluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
- Assay Run Time: For greater sensitivity an overnight incubation is recommended, (16-18 hours) at 2-8°C. Alternatively, incubate for 2 hours at room temperature (20-25°C).
- Research Category: Endocrine
- Research Subcategory: Metabolism
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Other Notes
Sensitivity: Please see kit protocol for sensitive’s of individual biomarkers.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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Thomas Blum et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(30), 15236-15243 (2019-07-10)
Dopamine neurons of the hypothalamic arcuate nucleus (ARC) tonically inhibit the release of the protein hormone prolactin from lactotropic cells in the anterior pituitary gland and thus play a central role in prolactin homeostasis of the body. Prolactin, in turn
Tomohiro Terasaka et al.
Endocrinology, 160(8), 1999-2014 (2019-06-13)
Gonadotropin secretion, which is elicited by GnRH stimulation of the anterior pituitary gonadotropes, is a critical feature of reproductive control and the maintenance of fertility. In addition, activation of the GnRH receptor (GnRHR) regulates transcription and translation of multiple factors
Marina O Fernandez et al.
Endocrinology, 158(1), 121-133 (2016-11-15)
The peroxisome-proliferator activated receptor γ (PPARγ) is expressed in the hypothalamus in areas involved in energy homeostasis and glucose metabolism. In this study, we created a deletion of PPARγ brain-knockout (BKO) in mature neurons in female mice to investigate its
Amanda Rodriguez et al.
Development (Cambridge, England), 146(23) (2019-11-11)
The number and quality of oocytes within the ovarian reserve largely determines fertility and reproductive lifespan in mammals. An oocyte-specific transcription factor cascade controls oocyte development, and some of these transcription factors, such as newborn ovary homeobox gene (NOBOX), are
An essential role for insulin and IGF1 receptors in regulating sertoli cell proliferation, testis size, and FSH action in mice.
Pitetti, JL; Calvel, P; Zimmermann, C; Conne, B; Papaioannou, MD; Aubry, F; Cederroth et al.
Molecular Endocrinology null
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