MABS1954

Anti-DARC (CD234) Antibody

• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: NaM185-2C3, monoclonal
• mol wt: calculated mol wt 35.55 kDa
• species reactivity: human
• packaging: antibody small pack of 100 μg
• technique(s): ELISA: suitable; flow cytometry: suitable; inhibition assay: suitable; radioimmunoassay: suitable; western blot: suitable
• isotype: IgG1κ
• UniProt accession no.: Q16570
• shipped in: dry ice
• storage temp.: -10 to -25°C
• target post-translational modification: unmodified
• Gene Information: human ... CKR1(2532)

Description

General description

Atypical chemokine receptor 1 (UniProt: Q16570; also known as Duffy antigen/chemokine receptor, DARC, Fy glycoprotein, GpFy, Glycoprotein D, Plasmodium vivax receptor, CD234) is encoded by the ACKR1 (also known as DARC, FY, GPD) gene (Gene ID: 2532) in human. DARC is a multi-pass membrane protein of the G-protein coupled receptor 1 family with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. Its N-terminal glycosylated extracellular domain carries the Duffy blood group antigens Fya and Fyb. It is present mainly on erythrocytes and endothelial cells of post-capillary venules of various tissues. DARC serves as a promiscuous receptor for a number of pro-inflammatory CC and CXC chemokines but lacks signal transduction ability. However, it controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. It is reported to regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms. When expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes. However, when expressed in erythrocytes, it serves as blood reservoir of cognate chemokines and a chemokine sink, buffering potential surges in plasma chemokine levels. Clone NaM185-2C3 recognizes a linear epitope, the essential portion of which is localized in amino acids 22-26 where all amino acid residues of the epitope, except Asp, are essential for antibody-binding. (Ref.: Grodecka, M., et al. (2010). Acta Biochimica Polonica. 57(1); 49-53; Wasniowska, K., et al. (2002). Transfusion Med. 12(3); 205-211).

Specificity

Clone NaM185-2C3 (2C3) is a mouse monoclonal antibody that detects DARC (CD234). It targets an epitope within 8 amino acids from the N-terminal region.

Immunogen

CHO cells transfected with cDNA encoding full-length Duffy antigen/chemokine receptor (DARC) protein.

Application

Quality Control Testing

Evaluated by Flow Cytometry in K562 cells.

Flow Cytometry Analysis: 1 μg of this antibody detected DARC (CD234) in one million K562 cells.

Tested Applications

Radioimmunoassay: A representative lot detected DARC (CD234) in Radioimmunoassay applications (Wasniowska, K., et. al. (2002). Transfus Med. 12(3):205-11).

Western Blotting Analysis: A representative lot detected DARC (CD234) in Western Blotting applications (Grodecka, M., et. al. (2010). Acta Biochim Pol. 57(1):49-53).

Flow Cytometry Analysis: A representative lot detected DARC (CD234) in Flow Cytometry applications (Menard, D., et. al. (2010). Proc Natl Acad Sci USA. 107(13):5967-71; Chi, J.S., et. al. (2016). Int J Parasitol. 46(1):31-9).

ELISA Analysis: A representative lot detected DARC (CD234) in ELISA applications (Wasniowska, K., et. al. (2002). Transfus Med. 12(3):205-11).

Inhibition Assay: A representative lot of this antibody blocked CXCL8 binding site on endothelial cells. (Whittall, C., et al. (2013). J. Immunol. 190(4):1725-36).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-DARC (CD234), clone NaM185-2C3, Cat. No. MABS1954, is a mouse monoclonal antibody that detects DARC (Duffy antigen/chemokine receptor) and is tested for use in ELISA, Flow Cytometry, Inhibition Assay, Radioimmunoassay, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Storage and Stability

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable