MABN540
Anti-mGluR5 Antibody, clone N75/33
clone N75/33, from mouse
Synonym(s):
Metabotropic glutamate receptor 5, mGluR5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
N75/33, monoclonal
species reactivity
human, mouse, rat
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GRM5(2915)
General description
Metabotropic glutamate receptor 5 (mGluR5) is a G-protein mediated glutamate receptor that generates a calcium-activated chloride current. mGluR5 is widely distributed in neuronal cells of the central nervous system.
Specificity
This antibody recognizes the C-terminus of mGluR5. This antibody may cross react with mGluR1 based upon <35% sequence homology.
Immunogen
Epitope: C-terminus
Recombinant protein corresponding to the C-terminus of rat mGluR5.
Application
Detect mGluR5 using this mouse monoclonal antibody, Anti-mGluR5 Antibody, clone N75/33 validated for use in western blotting & IHC.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
Western Blotting Analysis: 1 µg/mL from a representative lot detected mGluR5 in 10 µg of rat brain membrane tissue.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected mGluR5 in normal human midbrain tissue.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected mGluR5 in normal human midbrain tissue.
Quality
Evaluated by Western Blotting in mouse hypothalamus tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected mGluR5 in 10 µg of mouse hypothalamus tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected mGluR5 in 10 µg of mouse hypothalamus tissue lysate.
Target description
~150-270 kDa observed. This protein may be observed at higher molecular weights due to glycosylation (Bhave, G., et al. (2003). J Biol Chem. 278(32):30294-30301). This protein is observed as a dimer at ~270 kDa, but is observed as a monomer at ~140 kDa under reduced conditions in SDS-PAGE (Romano, C., et al. (1996). J Biol Chem. 271(45):28612-28616.). Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Hypothalamus tissue lysate
Hypothalamus tissue lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Cameron W Morris et al.
Biological psychiatry, 93(11), 976-988 (2023-02-24)
Grooming dysfunction is a hallmark of the obsessive-compulsive spectrum disorder trichotillomania. Numerous preclinical studies have utilized SAPAP3-deficient mice for understanding the neurobiology of repetitive grooming, suggesting that excessive grooming is caused by increased metabotropic glutamate receptor 5 (mGluR5) activity in
Wilber Romero-Fernandez et al.
Molecular neurobiology, 59(10), 5955-5969 (2022-07-14)
The adenosine A2A receptor (A2AR), dopamine D2 receptor (D2R) and metabotropic glutamate receptor type 5 (mGluR5) form A2AR-D2R-mGluR5 heteroreceptor complexes in living cells and in rat striatal neurons. In the current study, we present experimental data supporting the view that
Ming Chen et al.
Molecular neurodegeneration, 16(1), 14-14 (2021-03-06)
Before the deposition of amyloid-beta plaques and the onset of learning memory deficits, patients with Alzheimer's disease (AD) experience olfactory dysfunction, typified by a reduced ability to detect, discriminate, and identify odors. Rodent models of AD, such as the Tg2576
Caesar M Hernandez et al.
eNeuro, 5(3) (2018-07-05)
Glutamate signaling is essential for the persistent neural activity in prefrontal cortex (PFC) that enables working memory. Metabotropic glutamate receptors (mGluRs) are a diverse class of proteins that modulate excitatory neurotransmission via both presynaptic regulation of extracellular glutamate levels and
Qingwei Huo et al.
Cerebral cortex (New York, N.Y. : 1991), 27(8), 4060-4072 (2016-08-25)
Genetic and biochemical studies have focused on the role of amyloid β protein in the pathogenesis of Alzheimer's disease. In comparison, the physiological roles of its precursor protein, amyloid precursor protein (APP), in synaptic and network activity is less well studied.
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