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MAB1935

Sigma-Aldrich

Anti-Fibronectin Antibody, CT, clone 868A11

clone 868A11, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100
300

antibody form

purified antibody

antibody product type

primary antibodies

clone

868A11, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

Specificity

The C-terminal domain which contains the second fibrin binding site and it distinguishes between two major proteolytic fragments (140kd and 160dk), but not between the A and B chains. Epitope = C-terminal heparin binding fragment.

Immunogen

Epitope: C-terminus
Human plasma fibronectin.

Application

ELISA

Immunohistochemistry, formalin fixed, 10-25 μg/ml

Western blotting at 1-5 μg/ml

Final working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Fibronectin Antibody, C-terminus, clone 868A11 is validated for use in ELISA, WB for the detection of Fibronectin.

Quality

Tested

Physical form

Format: Purified
In 0.02MPB with 0.25M NaCl, pH=7.6 with 0.1% Sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C.

Other Notes

Please refer to lot specific datasheet.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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André N Tiaden et al.
The Journal of biological chemistry, 287(25), 21335-21345 (2012-05-05)
Human HTRA1 is a highly conserved secreted serine protease that degrades numerous extracellular matrix proteins. We have previously identified HTRA1 as being up-regulated in osteoarthritic patients and as having the potential to regulate matrix metalloproteinase (MMP) expression in synovial fibroblasts
Janette K Burgess et al.
The Journal of allergy and clinical immunology, 118(3), 649-657 (2006-09-05)
Airway smooth muscle (ASM) cells may contribute to airway remodeling through the release of growth factors, cytokines, and extracellular matrix (ECM) proteins. The effect of current asthma therapies on this release is not known. We examined the effect of corticosteroids
Brant Hubbard et al.
Matrix biology : journal of the International Society for Matrix Biology, 34, 124-131 (2013-10-24)
Extracellular matrix (ECM) conformation is regulated by a variety of stimuli in vivo, including mechanical forces and allosteric binding partners, and these conformational changes contribute to the regulation of cell behavior. Heparin and heparan sulfate, for example, have been shown
Definition of the native and denatured type II collagen binding site for fibronectin using a recombinant collagen system.
An, B; Abbonante, V; Yigit, S; Balduini, A; Kaplan, DL; Brodsky, B
The Journal of Biological Chemistry null
Binding and orientation of fibronectin on surfaces with collagen-related peptides. Student Research Award in the Masters Science Degree Candidate category, 27th annual meeting of the Society for Biomaterials, St. Paul, MN, April 24-29, 2001.
U Klueh, S Goralnick, J D Bryers, D L Kreutzer
Journal of Biomedical Materials Research null

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