HADCYMAG-61K

MILLIPLEX^® Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay

The Human Adipocyte Magnetic Bead Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 11 Adipocyte protein biomarkers in cell culture samples.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 100%; (IL-6); accuracy: 102%; (TNFα); accuracy: 103%; (MCP-1); accuracy: 83%; (PAI-1 (total)); accuracy: 99%; (HGF); sensitivity: 0.2 pg/mL; (MinDC+2SD; IL-8); sensitivity: 0.2 pg/mL; (MinDC+2SD; NGF); sensitivity: 0.2 pg/mL; (MinDC+2SD; TNF?); sensitivity: 0.6 pg/mL; (MinDC+2SD; IL-6); sensitivity: 10 pg/mL; (MinDC+2SD; Leptin); sensitivity: 15 pg/mL; (MinDC+2SD; Adiponectin); sensitivity: 2.0 pg/mL; (MinDC+2SD; IL-1?); sensitivity: 2.1 pg/mL; (MinDC+2SD; MCP-1); sensitivity: 3.0 pg/mL; (MinDC+2SD; HGF); sensitivity: 4.4 pg/mL; (MinDC+2SD; Resistin); sensitivity: 4.6 pg/mL; (MinDC+2SD; PAI-1 (total)); standard curve range: 0.29-4,000 pg/mL; (IL-8); standard curve range: 0.32-5,000 pg/mL; (NGF); standard curve range: 0.38-6,000 pg/mL; (TNFα); standard curve range: 0.51-8,000 pg/mL; (IL-1β); standard curve range: 0.96-150,000 pg/mL; (IL-6); standard curve range: 0.96-150,000 pg/mL; (MCP-1); standard curve range: 26-400,000 pg/mL; (Adipnectin); standard curve range: 6.4-100,000 pg/mL; (HGF); standard curve range: 6.4-100,000 pg/mL; (Resistin); standard curve range: 9.6-150,000 pg/mL; (Leptin); standard curve range: 9.6-150,000 pg/mL; (PAI-1); inter-assay cv: <15%; intra-assay cv: <10%; (Adiponectin); inter-assay cv: <15%; intra-assay cv: <10%; (IL-8); inter-assay cv: <15%; intra-assay cv: <10%; (MCP-1); inter-assay cv: <15%; intra-assay cv: <10%; (Resistin); inter-assay cv: <20%; intra-assay cv: <10%; (HGF); inter-assay cv: <20%; intra-assay cv: <10%; (IL-6); inter-assay cv: <20%; intra-assay cv: <10%; (NGF); inter-assay cv: <20%; intra-assay cv: <10%; (PAI-1 (total)); inter-assay cv: <20%; intra-assay cv: <10%; (TNFα); inter-assay cv: <20%; intra-assay cv: <15%; (IL-1β); inter-assay cv: <25%; intra-assay cv: <20%; (Leptin)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

The role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Adiponectin also has anti-inflammatory effects on the cellular lining of blood vessel walls which may help explain the association of high levels with reduced risk of heart attack.

The MILLIPLEX^® Human Adipocyte Panel is an 11-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cell/tissue culture samples: Adiponectin, HGF, IL-β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (total), Resistin, TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

• Analytes: Adiponectin, HGF, IL-1β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (Total), Resistin, TNF-α
• Recommended Sample Type: Tissue/cell culture supernatants or extracts
• Recommended Sample Dilution: 25 μL per well of undiluted tissue/cell culture sample; samples may require dilution with an appropriate medium prior to assay
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• Research Category: Metabolism
• Research Subcategory: Endocrine

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate

Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H411
• Precautionary Statements: P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects