CP01

Anti-Actin (Ab-1) Mouse mAb (JLA20)

liquid, clone JLA20, Calbiochem^®

• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: JLA20, monoclonal
• Species reactivity: -
• Application: —
• Citations: 218

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: JLA20, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)
• species reactivity (predicted by homology): all
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• dilution: (Immunoblotting (1:5000-1:20000); Immunofluorescence (1:10000, ; ; Recomended dilution for secondary antibody (0.05 µg/mL, 1:2000 dilution))
• isotype: IgM
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTB(60)

Description

General description

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.

Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.

Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.

Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1

Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN

Entrez Gene Name: actin, alpha 1, skeletal muscle

Hu Entrez ID: 58, 71 (Related Antibodies: CP64)

Mu Entrez ID: 11459 ;-; 11465

Rat Entrez ID: 29437 ;-; 287876

Immunogen

Chicken

chicken cytoskeletal proteins

Application

Immunoblotting (1:5000-1:20000)

Immunofluorescence (1:10000, see application references)



Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

Physical form

100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

Preparation Note

Following initial thaw, aliquot ascites and freeze (-20°C).

Analysis Note

Positive Control
All animal cell lines

Other Notes

Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.

Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable