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Key Documents

CBL198

Sigma-Aldrich

Anti-Cytokeratin 19 Antibody, clone BA 17

clone BA 17, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100
300

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

BA 17, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... KRT19(3880)

Specificity

The antibody is specific to human cytokeratin 19 and is reactive with cells of epithelial origin. Cytokeratin 19 is the smallest human cytokeratin (40 kDa) and is found in many simple and invariably in some non-keratinising stratified squamous epithelia. All adenocarcinomas tested have been positive for cytokeratin 19.

FUSION PARTNER: Px63 Ag 8.653 myeloma cell line

Immunogen

Human reduction mammoplasty organoids

Application

Detect Cytokeratin 19 using this Anti-Cytokeratin 19 Antibody, clone BA 17 validated for use in WB, IC, IH(P).
Immunocytochemistry

Western blotting

Suitable for staining frozen and paraffin wax embedded tissue sections after proteolytic enzyme digestion

Cytokeratin 19 positive detection distinguishes malignant from benign breast tumours

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Cytokeratins

Linkage

Replaces: CBL178

Physical form

Format: Purified
The monoclonal is presented at a concentration of 0.1mg/1ml in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Storage and Stability

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours.
Bartek, J, et al.
International Journal of Cancer. Journal International Du Cancer, 36, 299-306 (1985)
Gowher Ali et al.
STAR protocols, 3(3), 101613-101613 (2022-08-23)
The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with

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