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401442

Sigma-Aldrich

Goat Anti-Human IgG, γ-Chain Specific Alkaline Phosphatase Conjugate

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

General description

Immunoaffinity purified goat polyclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG γ-chain.
This Goat Anti-Human IgG, γ-Chain Specific Alk-Phos Conjugate is validated for use in Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgG, γ-Chain Specific.

Immunogen

Human

Application

Enzyme Immunoassay (1:5000)

Immunoelectrophoresis (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris-HCl, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, pH 8.0.

Other Notes

Monospecific for human IgG γ-chain as determined by immunoelectrophoresis against normal human serum. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pauline Versteegen et al.
Frontiers in immunology, 13, 864674-864674 (2022-06-10)
Immunogenicity of acellular pertussis (aP) vaccines is conventionally assessed by measuring antibody responses but antibody concentrations wane quickly after vaccination. Memory B cells, however, are critical in sustaining long-term protection and therefore may be an important factor when assessing pertussis
Darren Suryawijaya Ong et al.
Nature communications, 15(1), 6968-6968 (2024-08-14)
The use of pneumococcal conjugate vaccine (PCV) schedules with fewer doses are being considered to reduce costs and improve access, particularly in low- and middle-income countries. While several studies have assessed their immunogenicity, there are limited data on their potential
Aapo Knuutila et al.
Emerging microbes & infections, 12(1), 2204146-2204146 (2023-04-16)
Immunization during pregnancy (IP) against pertussis is recommended in many countries to protect infants. Although maternal antibodies can influence the infants' antibody responses to primary vaccinations, their effect on the development of functional antibodies and B cells remain poorly studied.
Jordan R Barrett et al.
Frontiers in immunology, 14, 1193079-1193079 (2024-02-01)
We have previously reported primary endpoints of a clinical trial testing two vaccine platforms for the delivery of Plasmodium vivax malaria DBPRII: viral vectors (ChAd63, MVA), and protein/adjuvant (PvDBPII with 50µg Matrix-M™ adjuvant). Delayed boosting was necessitated due to trial
Malick M Gibani et al.
Nature medicine, 25(7), 1082-1088 (2019-07-05)
Salmonella Typhi is a human host-restricted pathogen that is responsible for typhoid fever in approximately 10.9 million people annually1. The typhoid toxin is postulated to have a central role in disease pathogenesis, the establishment of chronic infection and human host

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