The solubility is not determined for this colloidal cellulose, and there is no specific protocol for using it in plaque assays. Based on literature, this product has been used in plaque assays at a concentration of 0.6% or 1.2% in serum-free DMEM.
435244
Cellulose
colloidal, microcrystalline
Synonym(s):
Cellulose powder, Cotton linters
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form
colloidal, microcrystalline
Quality Level
contains
10.0-20.0% sodium carboxymethyl cellulose as stabilizer
particle size
+65-+325 mesh
viscosity
50-150 cP, 2.6 wt. % in H2O, Brookfield RTV, spindle #1 (20 rpm)(lit.)
bulk density
0.6 g/mL (25°C)
SMILES string
O1[C@H](C(C(C(C1CO)O)O)O)O[C@@H]2C(OC(C(C2O)O)O)CO
InChI
1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3?,4?,5?,6?,7?,8?,9?,10-,11?,12+/m1/s1
InChI key
GUBGYTABKSRVRQ-WFVLMXAXSA-N
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Application
Bulking agent, opacifier, anti-caking agent, extrusion aid and stabilizer for foams and emulsions.
High purity cellulose powders for partition chromatography.
Features and Benefits
Amorphous regions are hydrolyzed leaving crystalline microfibrils. Forms thixotropic gels, good thermal stability.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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I am trying to use this an overlay medium for plaque assays- I tried dissolving in water but there are still fine particulates. How would I dissolve this or what media should I dissolve this in for complete dissolution?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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