- Expression of single-chain Fv fragments in E. coli cytoplasm.
Expression of single-chain Fv fragments in E. coli cytoplasm.
Methods in molecular biology (Clifton, N.J.) (2009-06-26)
Laurence Guglielmi, Pierre Martineau
PMID19554299
ZUSAMMENFASSUNG
The most frequently used approach to produce single-chain Fv fragments (scFv) and Fab in Escherichia coli is to express them in the periplasm of the bacteria. We present here an alternative procedure that uses cytoplasmic expression of soluble active scFv. This can be accomplished by using either specially engineered E. coli strains or hyperstable scFvs.
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Monoklonaler Anti-Polyhistidin−Peroxidase-Antikörper der Maus in Maus hergestellte Antikörper, clone HIS-1, purified from hybridoma cell culture