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Merck

A physical sciences network characterization of circulating tumor cell aggregate transport.

American journal of physiology. Cell physiology (2015-03-20)
Michael R King, Kevin G Phillips, Annachiara Mitrugno, Tae-Rin Lee, Adelaide M E de Guillebon, Siddarth Chandrasekaran, Matthew J McGuire, Russell T Carr, Sandra M Baker-Groberg, Rachel A Rigg, Anand Kolatkar, Madelyn Luttgen, Kelly Bethel, Peter Kuhn, Paolo Decuzzi, Owen J T McCarty
ZUSAMMENFASSUNG

Circulating tumor cells (CTC) have been implicated in the hematogenous spread of cancer. To investigate the fluid phase of cancer from a physical sciences perspective, the multi-institutional Physical Sciences-Oncology Center (PS-OC) Network performed multidisciplinary biophysical studies of single CTC and CTC aggregates from a patient with breast cancer. CTCs, ranging from single cells to aggregates comprised of 2-5 cells, were isolated using the high-definition CTC assay and biophysically profiled using quantitative phase microscopy. Single CTCs and aggregates were then modeled in an in vitro system comprised of multiple breast cancer cell lines and microfluidic devices used to model E-selectin mediated rolling in the vasculature. Using a numerical model coupling elastic collisions between red blood cells and CTCs, the dependence of CTC vascular margination on single CTCs and CTC aggregate morphology and stiffness was interrogated. These results provide a multifaceted characterization of single CTC and CTC aggregate dynamics in the vasculature and illustrate a framework to integrate clinical, biophysical, and mathematical approaches to enhance our understanding of the fluid phase of cancer.

MATERIALIEN
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DAPI, for nucleic acid staining
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Calciumchlorid -Lösung, BioUltra, for molecular biology, ~1 M in H2O
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EN300-127045-250MG250 mg
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EN300-127045-500MG500 mg
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286,80 €
EN300-127045-1G1 g
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354,40 €
EN300-127045-2.5G2.5 g
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572,80 €
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936,80 €
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Calciumchlorid, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
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152,90 €
EN300-127045-250MG250 mg
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198,40 €
EN300-127045-500MG500 mg
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286,80 €
EN300-127045-1G1 g
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354,40 €
EN300-127045-2.5G2.5 g
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572,80 €
EN300-127045-5G5 g
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936,80 €
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Calciumchlorid, anhydrous, powder, 99.99% trace metals basis
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EN300-127045-500MG500 mg
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286,80 €
EN300-127045-1G1 g
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354,40 €
EN300-127045-2.5G2.5 g
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572,80 €
EN300-127045-5G5 g
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936,80 €
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Calciumchlorid, AnhydroBeads, −10 mesh, ≥99.9% trace metals basis
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152,90 €
EN300-127045-250MG250 mg
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198,40 €
EN300-127045-500MG500 mg
Versand in 14 Tagen - ab Aldrich Partner.
286,80 €
EN300-127045-1G1 g
Versand in 14 Tagen - ab Aldrich Partner.
354,40 €
EN300-127045-2.5G2.5 g
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572,80 €
EN300-127045-5G5 g
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936,80 €
EN300-127045-10G10 g
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1.664,80 €
Sigma-Aldrich
Calciumchlorid Dihydrat
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EN300-127045-100MG100 mg
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152,90 €
EN300-127045-250MG250 mg
Versand in 14 Tagen - ab Aldrich Partner.
198,40 €
EN300-127045-500MG500 mg
Versand in 14 Tagen - ab Aldrich Partner.
286,80 €
EN300-127045-1G1 g
Versand in 14 Tagen - ab Aldrich Partner.
354,40 €
EN300-127045-2.5G2.5 g
Versand in 14 Tagen - ab Aldrich Partner.
572,80 €
EN300-127045-5G5 g
Versand in 14 Tagen - ab Aldrich Partner.
936,80 €
EN300-127045-10G10 g
Versand in 14 Tagen - ab Aldrich Partner.
1.664,80 €
Sigma-Aldrich
Calciumchlorid, AnhydroBeads, −10 mesh, ≥99.99% trace metals basis
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
SKULieferanten-SKUPackungsgrößeVerfügbarkeitPreisMenge
EN300-127045-100MG100 mg
Versand in 14 Tagen - ab Aldrich Partner.
152,90 €
EN300-127045-250MG250 mg
Versand in 14 Tagen - ab Aldrich Partner.
198,40 €
EN300-127045-500MG500 mg
Versand in 14 Tagen - ab Aldrich Partner.
286,80 €
EN300-127045-1G1 g
Versand in 14 Tagen - ab Aldrich Partner.
354,40 €
EN300-127045-2.5G2.5 g
Versand in 14 Tagen - ab Aldrich Partner.
572,80 €
EN300-127045-5G5 g
Versand in 14 Tagen - ab Aldrich Partner.
936,80 €
EN300-127045-10G10 g
Versand in 14 Tagen - ab Aldrich Partner.
1.664,80 €