G005669

HPLC Analysis of Aswagandha Extract on Discovery^® C18

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: column: Discovery C18, 25 cm x 4.6 mm I.D., 5 μm particles (504971); mobile phase: [A] 1 mM potassium phosphate, monobasic; [B] acetonitrile; gradient: 5 to 45% B in 18 min; 45 to 80% B in 7 min; held at 80% B for 3 min; flow rate: 1.5 mL/min; pressure: 3191 psi (220 bar); column temp.: 27 °C; detector: UV, 227 nm; injection: 20 μL; sample: 20 μg/mL in water: acetonitrile (86:14)
• Eignung: application for HPLC
• Anwendung(en): vitamins, nutraceuticals, and natural products

Beschreibung

Hinweis zur Analyse

This application demonstrates the suitability of Discovery C18 for the efficient separation of withania extract by the USP monograph.

Rechtliche Hinweise

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyt

Withanoside IV
Withanoside V
Withaferin A
12-Deoxywithastramonolide
Withanolide A
Withanolide B