SRP4135

IL-3 from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): HCGF P-cell stimulation factorIL-3 MGC79398, Interleukin-3, MCGF (Mast cell growth factor)Multi-CSF, MGC79399
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00164817

Eigenschaften

• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC), ≥98% (SDS-PAGE)
• form: lyophilized
• potency: <0.05 ng/mL ED₅₀
• mol wt: ~15.1 kDa
• packaging: pkg of 10 μg
• impurities: endotoxin, tested
• NCBI accession no.: P01586
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Il3(16187)

Beschreibung

General description

IL3 (interleukin 3) is an immunomodulatory cytokine and a multilineage hematopoietic growth factor. It is predominantly produced by activated CD4⁺ T cells and mast cells. Recombinant mouse IL-3 is a 15.1 kDa protein containing 135 amino acid residues.

Biochem/physiol Actions

IL3 (interleukin 3) was originally known as multicolony-stimulating factor due to its capability to promote the development of a wide variety of hematopoietic progenitor cells from bone marrow, such as basophils, neutrophils, eosinophils, erythrocytes, megakaryocytes, mast cells, macrophages, and dendritic cells. In vitro this protein facilitates murine hematopoietic progenitor cell differentiation, and the survival and activation of mature myeloid cells. It promotes the release of histamine and mast cell growth, differentiation, and mediator release, and hence, is implicated in allergic inflammation. It exhibits bi-functionality during osteoclastogenesis where it stimulates the development of osteoclast progenitors but suppresses the osteoclastogenic process. Certain variants in this gene confer protection against malaria attack, and thus, this gene might play a role in the pathophysiology of falciparum malaria.

Physical form

Sterile filtered and lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1 –1.0 mg/mL. The solution can then be diluted into other aqueous buffers.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable