SHC012

MISSION^® pLKO.1-puro-CMV-TagRFP^™ Positive Control Plasmid DNA

Contains a gene encoding TagRFP

• Synonym(e): MISSION^® Control Vectors
• MDL-Nummer: MFCD07785395
• UNSPSC-Code: 41106609
• NACRES: NA.51

Eigenschaften

• Qualitätsniveau: 200
• Produktlinie: MISSION^®
• Konzentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The CMV-TagRFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Anwendung

To see more application data, protocols, vector maps visit sigma.com/shrna.
The MISSION TagRFP Control Vector is an 8594 base pair lentivirus plasmid vector that contains a gene encoding TagRFP, under the control of the CMV promoter. The CMV-TagRFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.

Rechtliche Hinweise

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TagRFP is a trademark of Evrogen Co.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable