SAE0092
Mutanolysin aus Streptomyces globisporus ATCC 21553
free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)
Synonym(e):
Mutanolysin
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About This Item
Empfohlene Produkte
Biologische Quelle
Streptomyces sp. (Streptomyces globisporus ATCC 21553)
Form
lyophilized powder
Spezifische Aktivität
≥4000 units/mg protein (biuret)
Mol-Gew.
23 kDa
~18.5 kDa
Leistungsmerkmale
DNA free
Konzentration
≥40% protein
Methode(n)
nucleic acid detection: suitable
Eignung
suitable for cell lysis
Anwendung(en)
life science and biopharma
Versandbedingung
wet ice
Lagertemp.
−20°C
Anwendung
Es bietet eine sanfte Zelllyse zur Isolation von leicht abbaubaren Biomolekülen und RNA aus Bakterien. Es wird zur Bildung von Sphäroblasten zur Isolation von DNA eingesetzt.
Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Biochem./physiol. Wirkung
Mutanolysin ist eine N-Acetylmuramidase. Wie auch Lysozym ist es ein muralytisches Enzym, das die β-N-Acetylmuramyl-(1→4)-N-acetylglucosaminbindung des Bakterienzellwandpolymers Peptidoglykan-Polysaccharid spaltet. Seine carboxyterminalen Molekülteile sind an der Erkennung und Bindung von einzigartigen Zellwandpolymeren beteiligt. Mutanolysin lysiert Listeria und andere grampositive Bakterien wie Lactobacillus und Lactococcus.
Leistungsmerkmale und Vorteile
Suitable for difficult-to-lyse samples.
Microbial DNA free, minimizing DNA contamination from reagents in workflow.
Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
Microbial DNA free, minimizing DNA contamination from reagents in workflow.
Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
Einheitendefinition
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
Physikalische Form
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
Rechtliche Hinweise
Ficoll is a registered trademark of Cytiva
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls
Jennifer A Taylor et al.
Current protocols, 1(4), e80-e80 (2021-04-13)
The bacterial cell wall, composed of peptidoglycan (PG), provides structural integrity for the cell and is responsible for cell shape in most bacteria. Here we present tools to study the cell wall using a clickable PG-specific sugar, 2-alkyne muramic acid
Anna Cäcilia Ingham et al.
Applied and environmental microbiology, 87(18), e0122521-e0122521 (2021-07-01)
Drivers of pig trucks constitute a potential route of human transmission of livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398). In this study, we determined MRSA prevalence in pig truck drivers (n = 47) and monitored the nasal microbiota of 9
K Yokogawa et al.
Antimicrobial agents and chemotherapy, 6(2), 156-165 (1974-08-01)
Mutanolysin partially purified from the culture filtrate of Streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near pH 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. Mutanolysin exhibited maximal
Rebecca M Corrigan et al.
PLoS pathogens, 7(9), e1002217-e1002217 (2011-09-13)
The cell wall is a vital and multi-functional part of bacterial cells. For Staphylococcus aureus, an important human bacterial pathogen, surface proteins and cell wall polymers are essential for adhesion, colonization and during the infection process. One such cell wall
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